Values CatLog read from this paper
Characterization of the N-acetyl-α-D-glucosaminyl l-malate synthase and deacetylase functions for bacillithiol biosynthesis in Bacillus anthracis
PMID 20799687doi:10.1021/bi100698nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (9 values) from this paper: BaBshA (N-acetyl-α-D-glucosaminyl L-malate synthase, BshA glycosyltransferase), BaBshB (GlcNAc-malate deacetylase, BshB) from Bacillus anthracis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BaBshA (N-acetyl-α-D-glucosaminyl L-malate synthase, BshA glycosyltransferase) | Bacillus anthracis | L-malate | — | 0.058 | — | pH 7.5, 37 °C | Open record: BaBshA (N-acetyl-α-D-glucosaminyl L-malate synthase, BshA glycosyltransferase), L-malate |
| BaBshA (N-acetyl-α-D-glucosaminyl L-malate synthase, BshA glycosyltransferase) | Bacillus anthracis | UDP-GlcNAcHPLC assay of UDP formation (A260); 100 mM NaCl | 28 | 0.37 | 76 | pH 7.5, 37 °C | Open record: BaBshA (N-acetyl-α-D-glucosaminyl L-malate synthase, BshA glycosyltransferase), UDP-GlcNAc |
| BaBshA (N-acetyl-α-D-glucosaminyl L-malate synthase, BshA glycosyltransferase) | Bacillus anthracis | UDP-GlcNAc50 mM KCl; coupled assay: 7.5 U pyruvate kinase | 15 | 0.22 | 68 | pH 7.5, 37 °C | Open record: BaBshA (N-acetyl-α-D-glucosaminyl L-malate synthase, BshA glycosyltransferase), UDP-GlcNAc |
| BaBshB (GlcNAc-malate deacetylase, BshB) | Bacillus anthracis | N-acetyl-alpha-D-glucosaminyl L-malate | 42 | 0.16 | 260 calc. | pH 7.5, 37 °C | Open record: BaBshB (GlcNAc-malate deacetylase, BshB), N-acetyl-alpha-D-glucosaminyl L-malate |