CatLogenzyme kinetics catalog

Values CatLog read from this paper

Biochemical and thermodynamic characterization of mutated β1,4-galactosyltransferase 7 involved in the progeroid form of the Ehlers-Danlos syndrome

PMID 20809901doi:10.1042/bj20100921Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (41 values) from this paper: β1,4-galactosyltransferase 7 (β1,4-GalT7) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.1.133

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
β1,4-galactosyltransferase 7 (β1,4-GalT7)Homo sapiens4-methylumbelliferyl-β-D-xylopyranoside0.04-0.2 μM purified MBP-fusion protein—0.274.12pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)Homo sapiens4-methylumbelliferyl-β-D-xylopyranoside0.04-1 μM purified MBP-fusion protein—0.620.0457pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)Homo sapiens4-nitrophenyl-β-D-xylopyranosidemembrane fraction (0.3-0.6 g/l total protein); full-length—0.85—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)Homo sapiens4-nitrophenyl-β-D-xylopyranoside0.04-0.2 μM purified MBP-fusion protein; MBP fusion—1.271.32pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)Homo sapiensUDP-Galmembrane fraction (0.3-0.6 g/l total protein); full-length—0.29—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)Homo sapiensUDP-Gal0.04-0.2 μM purified MBP-fusion protein; MBP fusion1.110.234.82pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)Homo sapiensUDP-Gal0.04-1 μM purified MBP-fusion protein; cosubstrate 4MU-Xyl0.0280.80.0355pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant A186DHomo sapiens4-methylumbelliferyl-β-D-xylopyranoside—2.260.43pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant A186DHomo sapiens4-nitrophenyl-β-D-xylopyranosidemembrane fraction (0.3-0.6 g/l total protein); full-length—3.76—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant A186DHomo sapiens4-nitrophenyl-β-D-xylopyranoside0.04-0.2 μM purified MBP-fusion protein; MBP fusion—4.250.3pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant A186DHomo sapiensUDP-Galmembrane fraction (0.3-0.6 g/l total protein); full-length—2.41—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant A186DHomo sapiensUDP-Gal0.04-0.2 μM purified MBP-fusion protein; MBP fusion12.070.48pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant L206AHomo sapiens4-nitrophenyl-β-D-xylopyranoside—2.29—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant L206AHomo sapiensUDP-Gal—0.31—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant L206PHomo sapiens4-methylumbelliferyl-β-D-xylopyranoside—2.990.35pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant L206PHomo sapiens4-nitrophenyl-β-D-xylopyranoside—5.540.22pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant L206PHomo sapiensUDP-Gal1.060.651.63pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant R270CHomo sapiens4-methylumbelliferyl-β-D-xylopyranoside—3.260.32pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant R270CHomo sapiens4-nitrophenyl-β-D-xylopyranosidemembrane fraction (0.3-0.6 g/l total protein); full-length—5.01—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant R270CHomo sapiens4-nitrophenyl-β-D-xylopyranoside0.04-0.2 μM purified MBP-fusion protein; MBP fusion—7.930.13pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant R270CHomo sapiensUDP-Galmembrane fraction (0.3-0.6 g/l total protein); full-length—0.49—pH 7Open
β1,4-galactosyltransferase 7 (β1,4-GalT7)variant R270CHomo sapiensUDP-Gal0.04-0.2 μM purified MBP-fusion protein; MBP fusion1.050.631.7pH 7Open