Values CatLog read from this paper

Structural insights into substrate specificity in variants of N-acetylneuraminic Acid lyase produced by directed evolution

PMID 20826162doi:10.1016/j.jmb.2010.08.008Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 14 records (42 values) from this paper: N-acetylneuraminic acid lyase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.1.3.3

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
N-acetylneuraminic acid lyasevariant E192D	Escherichia coli	(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid	0.1	1.1	0.083	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192D, (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
N-acetylneuraminic acid lyasevariant E192D	Escherichia coli	N-acetylneuraminic acid	2	4.6	0.43	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192D, N-acetylneuraminic acid
N-acetylneuraminic acid lyasevariant E192F	Escherichia coli	(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid	1.47	0.3	5.2	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192F, (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
N-acetylneuraminic acid lyasevariant E192F	Escherichia coli	N-acetylneuraminic acid	2.67	5.9	0.45	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192F, N-acetylneuraminic acid
N-acetylneuraminic acid lyasevariant E192H	Escherichia coli	(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid	0.63	0.5	1.3	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192H, (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
N-acetylneuraminic acid lyasevariant E192H	Escherichia coli	N-acetylneuraminic acid	2	6.2	0.32	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192H, N-acetylneuraminic acid
N-acetylneuraminic acid lyasevariant E192M	Escherichia coli	(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid	1.17	0.2	5.3	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192M, (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
N-acetylneuraminic acid lyasevariant E192M	Escherichia coli	N-acetylneuraminic acid	1.17	5	0.23	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192M, N-acetylneuraminic acid
N-acetylneuraminic acid lyasevariant E192P	Escherichia coli	(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid	0.82	0.2	4.3	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192P, (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
N-acetylneuraminic acid lyasevariant E192P	Escherichia coli	N-acetylneuraminic acid	0.42	6	0.067	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192P, N-acetylneuraminic acid
N-acetylneuraminic acid lyasevariant E192Q	Escherichia coli	(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid	2.83	0.7	4.2	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192Q, (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
N-acetylneuraminic acid lyasevariant E192Q	Escherichia coli	N-acetylneuraminic acid	5	3.5	1.42	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192Q, N-acetylneuraminic acid
N-acetylneuraminic acid lyasevariant E192S	Escherichia coli	(5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid	0.43	0.6	0.7	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192S, (5R,6R)-7-(dipropylamino)-4,5,6-trihydroxy-2,7-dioxoheptanoic acid
N-acetylneuraminic acid lyasevariant E192S	Escherichia coli	N-acetylneuraminic acid	0.83	6	0.13	pH 7.5, 25 °C	Open record: N-acetylneuraminic acid lyase variant E192S, N-acetylneuraminic acid