Values CatLog read from this paper
Structural and kinetic characterization of 4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase, a protocatechuate degradation enzyme evolutionarily convergent with the HpaI…
PMID 20843800doi:10.1074/jbc.m110.159509Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (32 values) from this paper: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-carboxy-4-hydroxy-2-oxoadipate1.0 mM Mg2+ | 14.4 | 0.066 | 220 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-carboxy-4-hydroxy-2-oxoadipate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-carboxy-4-hydroxy-2-oxoadipate1.0 mM Mn2+ | 7.5 | 0.03 | 250 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-carboxy-4-hydroxy-2-oxoadipate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate1.0 mM Mg2+ | 19.3 | 0.26 | 74 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate1.0 mM Mn2+ | 13.7 | 0.022 | 620 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), 4-hydroxy-4-methyl-2-oxoglutarate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | oxaloacetate1.0 mM Mg2+ | 1.85 | 0.3 | 6.2 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), oxaloacetate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | oxaloacetate1.0 mM Mn2+ | 1.52 | 0.036 | 42 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), oxaloacetate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | racemic 4-hydroxy-2-oxopentanoate1.0 mM Mg2+ | 0.048 | 25 | 0.0019 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), racemic 4-hydroxy-2-oxopentanoate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | racemic 4-hydroxy-2-oxopentanoate1.0 mM Mn2+ | 0.19 | 8.8 | 0.022 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), racemic 4-hydroxy-2-oxopentanoate |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | racemic alpha-keto-gamma-hydroxyglutarate (4-hydroxy-2-oxoglutarate)1.0 mM Mg2+ | 0.058 | 0.15 | 0.38 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), racemic alpha-keto-gamma-hydroxyglutarate (4-hydroxy-2-oxoglutarate) |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) | Pseudomonas putida | racemic alpha-keto-gamma-hydroxyglutarate (4-hydroxy-2-oxoglutarate)1.0 mM Mn2+ | 0.25 | 0.071 | 3.5 | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase), racemic alpha-keto-gamma-hydroxyglutarate (4-hydroxy-2-oxoglutarate) |
| HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase)variant R123K | Pseudomonas putida | 4-hydroxy-4-methyl-2-oxoglutarate | 0.099 | 5.5 | 0.018 calc. | pH 8, 25 °C | Open record: HMG/CHA aldolase (4-hydroxy-4-methyl-2-oxoglutarate/4-carboxy-4-hydroxy-2-oxoadipate aldolase) variant R123K, 4-hydroxy-4-methyl-2-oxoglutarate |