CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of key functional residues in the active site of human β1,4-galactosyltransferase 7: a major enzyme in the glycosaminoglycan synthesis pathway

PMID 20843813doi:10.1074/jbc.m110.151951Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 27 records (37 values) from this paper: beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.1.133

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)Homo sapiens4-methylumbelliferyl beta-D-xylopyranoside1 h incubation; full-length; C-terminal Myc tag—0.32—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)Homo sapiens4-methylumbelliferyl beta-D-xylopyranosidepurified GST-β4GalT7 (0.2 µg); 30 min incubation1.790.335.42pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)Homo sapiensUDP-alpha-D-galactose1 h incubation; full-length; C-terminal Myc tag—0.31—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)Homo sapiensUDP-alpha-D-galactosepurified GST-β4GalT7 (0.2 µg); 30 min incubation1.520.285.43pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant D165EHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—1.76—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant D165EHomo sapiensUDP-alpha-D-galactose—1.17—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant D228EHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—2.57—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant D228EHomo sapiensUDP-alpha-D-galactose—0.47—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant E227DHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside1 h incubation; full-length; C-terminal Myc tag—0.52—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant E227DHomo sapiens4-methylumbelliferyl beta-D-xylopyranosidepurified GST-β4GalT7 (0.2 µg); 30 min incubation0.130.740.17pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant E227DHomo sapiensUDP-alpha-D-galactose1 h incubation; full-length; C-terminal Myc tag—0.47—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant E227DHomo sapiensUDP-alpha-D-galactosepurified GST-β4GalT7 (0.2 µg); 30 min incubation0.460.14.6pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant E230AHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—0.57—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant E230AHomo sapiensUDP-alpha-D-galactose—0.43—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant F221AHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—4.22—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant F221AHomo sapiensUDP-alpha-D-galactose—0.36—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant F221YHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—0.38—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant F221YHomo sapiensUDP-alpha-D-galactose—0.33—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant G223AHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—0.73—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant G223AHomo sapiensUDP-alpha-D-galactose—0.45—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant V164AHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—0.53—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant V164AHomo sapiensUDP-alpha-D-galactose—0.48—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant W222AHomo sapiensUDP-alpha-D-galactose—0.21—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant W222FHomo sapiens4-methylumbelliferyl beta-D-xylopyranoside—0.16—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant W222FHomo sapiensUDP-alpha-D-galactose—0.45—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant W224HHomo sapiensUDP-alpha-D-galactose1 h incubation; full-length; C-terminal Myc tag—0.07—pH 7, 37 °COpen
beta1,4-galactosyltransferase 7 (β4GalT7, xylosylprotein β1,4-galactosyltransferase)variant W224HHomo sapiensUDP-alpha-D-galactosepurified GST-β4GalT7 (0.2 µg); 30 min incubation0.10.025pH 7, 37 °COpen