Values CatLog read from this paper
Directed evolution of a thermostable quorum-quenching lactonase from the amidohydrolase superfamily
PMID 20980257doi:10.1074/jbc.m110.177139Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 32 records (74 values) from this paper: GKL (phosphotriesterase-like lactonase), MCP (phosphotriesterase-like lactonase) from Geobacillus kaustophilus, Mycobacterium avium subsp. paratuberculosis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-(3-oxo-dodecanoyl)-L-homoserine lactone | — | — | 0.13 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-(3-oxo-dodecanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-(3-oxo-hexanoyl)-L-homoserine lactone | — | — | 0.0045 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-(3-oxo-hexanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactone | 0.29 | 5.9 | 0.05 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-(3-oxo-octanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactone60 °C in 2.5 mM Bicine | — | — | 0.028 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-(3-oxo-octanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactone90 °C in 2.5 mM Bicine | — | — | 0.019 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-(3-oxo-octanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-decanoyl-DL-homoserine lactone | — | — | 0.3 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-decanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-hexanoyl-DL-homoserine lactone | 0.17 | 2.6 | 0.066 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-hexanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | N-octanoyl-DL-homoserine lactone | 0.52 | 1.2 | 0.43 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), N-octanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | Paraoxon | 0.0031 | 0.69 | 0.0045 | pH 8, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), Paraoxon |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | γ-Nonalactone | 2.9 | 0.024 | 120 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), γ-Nonalactone |
| GKL (phosphotriesterase-like lactonase) | Geobacillus kaustophilus | δ-Nonalactone | 29 | 0.035 | 840 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase), δ-Nonalactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | N-(3-oxo-dodecanoyl)-L-homoserine lactone | 2 | 0.48 | 4.2 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, N-(3-oxo-dodecanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | N-(3-oxo-hexanoyl)-L-homoserine lactone | 0.91 | 26 | 0.035 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, N-(3-oxo-hexanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactone | 1.1 | 3.8 | 0.28 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, N-(3-oxo-octanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | N-decanoyl-DL-homoserine lactone | 0.43 | 0.13 | 3.3 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, N-decanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | N-hexanoyl-DL-homoserine lactone | 0.2 | 2.4 | 0.082 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, N-hexanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | N-octanoyl-DL-homoserine lactone | 0.3 | 0.095 | 3.2 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, N-octanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | γ-Nonalactone | 22 | 1.1 | 21 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, γ-Nonalactone |
| GKL (phosphotriesterase-like lactonase)variant E101G/R230C | Geobacillus kaustophilus | δ-Nonalactone | — | — | 3.9 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101G/R230C, δ-Nonalactone |
| GKL (phosphotriesterase-like lactonase)variant E101K/R230P | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactone | — | — | 0.021 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101K/R230P, N-(3-oxo-octanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | N-(3-oxo-dodecanoyl)-L-homoserine lactone | 6.9 | 0.74 | 9.3 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, N-(3-oxo-dodecanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | N-(3-oxo-hexanoyl)-L-homoserine lactone | 0.4 | 9 | 0.045 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, N-(3-oxo-hexanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactoneTable 1 | 1.8 | 2.9 | 0.64 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, N-(3-oxo-octanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactoneTable 2 | — | — | 0.27 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, N-(3-oxo-octanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | N-decanoyl-DL-homoserine lactone | 1.7 | 0.13 | 13 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, N-decanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | N-hexanoyl-DL-homoserine lactone | 0.25 | 3.7 | 0.068 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, N-hexanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | N-octanoyl-DL-homoserine lactone | 1.4 | 0.39 | 3.7 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, N-octanoyl-DL-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | γ-Nonalactone | 2 | 0.46 | 4.3 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, γ-Nonalactone |
| GKL (phosphotriesterase-like lactonase)variant E101N/R230I | Geobacillus kaustophilus | δ-Nonalactone | 1.6 | 1.1 | 1.4 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101N/R230I, δ-Nonalactone |
| GKL (phosphotriesterase-like lactonase)variant E101V/R230T | Geobacillus kaustophilus | N-(3-oxo-hexanoyl)-L-homoserine lactone | — | — | 0.017 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101V/R230T, N-(3-oxo-hexanoyl)-L-homoserine lactone |
| GKL (phosphotriesterase-like lactonase)variant E101V/R230T | Geobacillus kaustophilus | N-(3-oxo-octanoyl)-L-homoserine lactone | — | — | 0.11 | pH 8.3, 37 °C | Open record: GKL (phosphotriesterase-like lactonase) variant E101V/R230T, N-(3-oxo-octanoyl)-L-homoserine lactone |
| MCP (phosphotriesterase-like lactonase)variant N266Y | Mycobacterium avium subsp. paratuberculosis | N-(3-oxo-octanoyl)-L-homoserine lactone | — | — | 9.9 | 25 °C | Open record: MCP (phosphotriesterase-like lactonase) variant N266Y, N-(3-oxo-octanoyl)-L-homoserine lactone |