Values CatLog read from this paper
Molybdopterin dinucleotide biosynthesis in Escherichia coli: identification of amino acid residues of molybdopterin dinucleotide transferases that determine specificity for binding of guanine or…
PMID 21081498doi:10.1074/jbc.m110.155671Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (22 values) from this paper: MobA (GTP:molybdopterin guanylyltransferase), MocA (CTP:molybdopterin cytidylyltransferase), MobA/MocA chimera and 1 more from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 11 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| MobA (GTP:molybdopterin guanylyltransferase) | Escherichia coli | guanosine 5'-triphosphate | 0.0048 | 0.0065 | 0.74 calc. | pH 7.2 | Open record: MobA (GTP:molybdopterin guanylyltransferase), guanosine 5'-triphosphate |
| MobA (GTP:molybdopterin guanylyltransferase)variant L12T/G14A (MobA-M1) | Escherichia coli | guanosine 5'-triphosphate | 0.0053 | 0.0489 | 0.11 calc. | pH 7.2 | Open record: MobA (GTP:molybdopterin guanylyltransferase) variant L12T/G14A (MobA-M1), guanosine 5'-triphosphate |
| MobA (GTP:molybdopterin guanylyltransferase)variant L12T/G14A/P79L/A81T/G82S (MobA-M1-M2) | Escherichia coli | cytidine 5'-triphosphate | 0.0015 | 0.031 | 0.048 calc. | pH 7.2 | Open record: MobA (GTP:molybdopterin guanylyltransferase) variant L12T/G14A/P79L/A81T/G82S (MobA-M1-M2), cytidine 5'-triphosphate |
| MobA (GTP:molybdopterin guanylyltransferase)variant L12T/G14A/P79L/A81T/G82S (MobA-M1-M2) | Escherichia coli | guanosine 5'-triphosphate | 0.0017 | 0.0455 | 0.037 calc. | pH 7.2 | Open record: MobA (GTP:molybdopterin guanylyltransferase) variant L12T/G14A/P79L/A81T/G82S (MobA-M1-M2), guanosine 5'-triphosphate |
| MobA (GTP:molybdopterin guanylyltransferase)variant P79L/A81T/G82S (MobA-M2) | Escherichia coli | guanosine 5'-triphosphate | 0.0042 | 0.0706 | 0.059 calc. | pH 7.2 | Open record: MobA (GTP:molybdopterin guanylyltransferase) variant P79L/A81T/G82S (MobA-M2), guanosine 5'-triphosphate |
| MobA/MocA chimerachimera N-MobA-C-MocA | Escherichia coli | guanosine 5'-triphosphate | 0.0073 | 0.0069 | 1.1 calc. | pH 7.2 | Open record: MobA/MocA chimera chimera N-MobA-C-MocA, guanosine 5'-triphosphate |
| MocA (CTP:molybdopterin cytidylyltransferase)variant L79P/T81A/S82G (MocA-M2) | Escherichia coli | cytidine 5'-triphosphate | 0.0043 | 0.0506 | 0.086 calc. | pH 7.2 | Open record: MocA (CTP:molybdopterin cytidylyltransferase) variant L79P/T81A/S82G (MocA-M2), cytidine 5'-triphosphate |
| MocA (CTP:molybdopterin cytidylyltransferase)variant T9L/A11G (MocA-M1) | Escherichia coli | cytidine 5'-triphosphate | 0.0018 | 0.0397 | 0.046 calc. | pH 7.2 | Open record: MocA (CTP:molybdopterin cytidylyltransferase) variant T9L/A11G (MocA-M1), cytidine 5'-triphosphate |
| MocA (CTP:molybdopterin cytidylyltransferase)variant T9L/A11G/L79P/T81A/S82G (MocA-M1-M2) | Escherichia coli | cytidine 5'-triphosphate | 0.002 | 0.04541 | 0.044 calc. | pH 7.2 | Open record: MocA (CTP:molybdopterin cytidylyltransferase) variant T9L/A11G/L79P/T81A/S82G (MocA-M1-M2), cytidine 5'-triphosphate |
| MocA (CTP:molybdopterin cytidylyltransferase)variant T9L/A11G/L79P/T81A/S82G (MocA-M1-M2) | Escherichia coli | guanosine 5'-triphosphate | 8.3 × 10⁻⁴ | 0.0361 | 0.023 calc. | pH 7.2 | Open record: MocA (CTP:molybdopterin cytidylyltransferase) variant T9L/A11G/L79P/T81A/S82G (MocA-M1-M2), guanosine 5'-triphosphate |
| MocA/MobA chimerachimera N-MocA-C-MobA | Escherichia coli | cytidine 5'-triphosphate | 0.0038 | 0.0056 | 0.68 calc. | pH 7.2 | Open record: MocA/MobA chimera chimera N-MocA-C-MobA, cytidine 5'-triphosphate |