Values CatLog read from this paper
Kinetics comparisons of mammalian Atg4 homologues indicate selective preferences toward diverse Atg8 substrates
PMID 21177865doi:10.1074/jbc.m110.199059Open in Browse
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CatLog read 20 records (38 values) from this paper: Atg4B, Atg4A, Atg4C and 1 more from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Atg4A | Homo sapiens | human GABA(A) receptor-associated protein (GABARAP) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0208 | 2.46 | pH 7.2, 37 °C | Open record: Atg4A, human GABA(A) receptor-associated protein (GABARAP) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4A | Homo sapiens | human GABA(A) receptor-associated protein-like 1 (Atg8L/GABARAPL1) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0367 | 1.96 | pH 7.2, 37 °C | Open record: Atg4A, human GABA(A) receptor-associated protein-like 1 (Atg8L/GABARAPL1) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4A | Homo sapiens | human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0157 | 12.8 | pH 7.2, 37 °C | Open record: Atg4A, human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4A | Homo sapiens | human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0339 | 0.0585 | pH 7.2, 37 °C | Open record: Atg4A, human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4B | Homo sapiens | human GABA(A) receptor-associated protein (GABARAP) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0058 | 78.9 | pH 7.2, 37 °C | Open record: Atg4B, human GABA(A) receptor-associated protein (GABARAP) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4B | Homo sapiens | human GABA(A) receptor-associated protein-like 1 (Atg8L/GABARAPL1) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0044 | 88.8 | pH 7.2, 37 °C | Open record: Atg4B, human GABA(A) receptor-associated protein-like 1 (Atg8L/GABARAPL1) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4B | Homo sapiens | human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0061 | 107 | pH 7.2, 37 °C | Open record: Atg4B, human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4B | Homo sapiens | human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0051 | 89.6 | pH 7.2, 37 °C | Open record: Atg4B, human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4B | Homo sapiens | N-acetylated octapeptide of LC3B residues 113-120 C-terminally linked to 7-amino-4-trifluoromethylcoumarin (AFC) | — | — | 4.7 × 10⁻⁵ | pH 7.2, 37 °C | Open record: Atg4B, N-acetylated octapeptide of LC3B residues 113-120 C-terminally linked to 7-amino-4-trifluoromethylcoumarin (AFC) |
| Atg4Bvariant C74S | Homo sapiens | human GABA(A) receptor-associated protein (GABARAP) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0084 | 0.0109 | pH 7.2, 37 °C | Open record: Atg4B variant C74S, human GABA(A) receptor-associated protein (GABARAP) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4Bvariant C74S | Homo sapiens | human GABA(A) receptor-associated protein-like 1 (Atg8L/GABARAPL1) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0035 | 0.0165 | pH 7.2, 37 °C | Open record: Atg4B variant C74S, human GABA(A) receptor-associated protein-like 1 (Atg8L/GABARAPL1) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4Bvariant C74S | Homo sapiens | human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0044 | 0.0659 | pH 7.2, 37 °C | Open record: Atg4B variant C74S, human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4Bvariant C74S | Homo sapiens | human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0068 | 0.0436 | pH 7.2, 37 °C | Open record: Atg4B variant C74S, human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4Bvariant Δ1–24 | Homo sapiens | human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0094 | 121 | pH 7.2, 37 °C | Open record: Atg4B variant Δ1–24, human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4Bvariant Δ1–24 | Homo sapiens | human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0059 | 118 | pH 7.2, 37 °C | Open record: Atg4B variant Δ1–24, human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4Bvariant Δ1–24 | Homo sapiens | N-acetylated octapeptide of LC3B residues 113-120 C-terminally linked to 7-amino-4-trifluoromethylcoumarin (AFC) | — | — | 6.1 × 10⁻⁵ | pH 7.2, 37 °C | Open record: Atg4B variant Δ1–24, N-acetylated octapeptide of LC3B residues 113-120 C-terminally linked to 7-amino-4-trifluoromethylcoumarin (AFC) |
| Atg4C | Homo sapiens | human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0124 | 0.0203 | pH 7.2, 37 °C | Open record: Atg4C, human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4C | Homo sapiens | human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0475 | 0.0122 | pH 7.2, 37 °C | Open record: Atg4C, human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4D | Homo sapiens | human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0072 | 0.0328 | pH 7.2, 37 °C | Open record: Atg4D, human GABA(A) receptor-associated protein-like 2 (GATE-16, Golgi-associated ATPase enhancer-16) with N-terminal His6 tag and C-terminal GST fusion |
| Atg4D | Homo sapiens | human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion | — | 0.0131 | 0.0342 | pH 7.2, 37 °C | Open record: Atg4D, human microtubule-associated protein 1A/1B light chain 3B (LC3B) with N-terminal His6 tag and C-terminal GST fusion |