CatLogenzyme kinetics catalog

Values CatLog read from this paper

Biochemical and structural characterization of WlbA from Bordetella pertussis and Chromobacterium violaceum: enzymes required for the biosynthesis of 2,3-diacetamido-2,3-dideoxy-D-mannuronic acid

PMID 21241053doi:10.1021/bi101871fOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 12 records (24 values) from this paper: WlbA (UDP-GlcNAcA 3-dehydrogenase) from Bordetella pertussis, Chromobacterium violaceum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.335

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
WlbA (UDP-GlcNAcA 3-dehydrogenase)Bordetella pertussisnicotinamide adenine dinucleotide (oxidized)initial velocity pattern (both substrates varied)—0.97—pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Bordetella pertussisnicotinamide adenine dinucleotide (oxidized)saturation kinetics (equation 2); 4.9 µM WlbA—0.055—pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Bordetella pertussisnicotinamide adenine dinucleotide (oxidized)saturation kinetics (equation 2); 14.4 µM WlbA—0.95—pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Bordetella pertussisUDP-D-glucuronic acid0.00212.050.001pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Bordetella pertussisUDP-N-acetyl-D-glucosaminuronic acidinitial velocity pattern (both substrates varied)0.00472.360.002pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Bordetella pertussisUDP-N-acetyl-D-glucosaminuronic acidsaturation kinetics (equation 2); 4.9 µM WlbA0.00390.0150.26pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Chromobacterium violaceumnicotinamide adenine dinucleotide (oxidized)initial velocity pattern (both substrates varied)—0.47—pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Chromobacterium violaceumnicotinamide adenine dinucleotide (oxidized)saturation kinetics (equation 2); 6.1 µM WlbA—0.089—pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Chromobacterium violaceumnicotinamide adenine dinucleotide (oxidized)saturation kinetics (equation 2); 23.0 µM WlbA—0.45—pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Chromobacterium violaceumUDP-D-glucuronic acid1.5 × 10⁻⁴0.27.5 × 10⁻⁴pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Chromobacterium violaceumUDP-N-acetyl-D-glucosaminuronic acidinitial velocity pattern (both substrates varied)3.1 × 10⁻⁴1.12.8 × 10⁻⁴pH 8.5, 25 °COpen
WlbA (UDP-GlcNAcA 3-dehydrogenase)Chromobacterium violaceumUDP-N-acetyl-D-glucosaminuronic acidsaturation kinetics (equation 2); 6.1 µM WlbA7.6 × 10⁻⁴0.00560.135pH 8.5, 25 °COpen