Values CatLog read from this paper
Enhanced expression and purification of fungal galactose oxidase in Escherichia coli and use for analysis of a saturation mutagenesis library
PMID 21264996doi:10.1002/cbic.201000634Open in Browse
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CatLog read 19 records (57 values) from this paper: galactose oxidase from Fusarium graminearum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D (MatGO-N6M1; the paper calls it wild type) | Fusarium graminearum | D-galactose | 1.1 × 10³ | 54 | 20 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D (MatGO-N6M1; the paper calls it wild type), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383A (C383A in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 1.2 × 10³ | 160 | 7.7 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383A (C383A in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383D (C383D in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 440 | 84 | 5.2 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383D (C383D in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383E (C383E in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 550 | 21 | 26 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383E (C383E in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383F (C383F in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 190 | 130 | 1.5 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383F (C383F in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383G (C383G in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 1.1 × 10³ | 83 | 14 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383G (C383G in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383H (C383H in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 210 | 30 | 7 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383H (C383H in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383I (C383I in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 260 | 510 | 0.51 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383I (C383I in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383K (C383K in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 1.1 × 10³ | 48 | 23 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383K (C383K in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383L (C383L in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 450 | 55 | 8.2 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383L (C383L in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383M (C383M in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 510 | 33 | 15 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383M (C383M in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383N (C383N in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 410 | 390 | 1.1 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383N (C383N in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383P (C383P in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 490 | 140 | 3.5 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383P (C383P in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383Q (C383Q in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 170 | 64 | 2.6 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383Q (C383Q in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383R (C383R in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 8.8 | 290 | 0.03 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383R (C383R in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383S (C383S in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 1.1 × 10³ | 34 | 32 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383S (C383S in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383T (C383T in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 3.4 × 10³ | 530 | 6.4 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383T (C383T in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383V (C383V in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 360 | 430 | 0.83 | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383V (C383V in the GO-N6M1 background), D-galactose |
| galactose oxidasevariant S10P/M70V/G195E/V494A/N535D/C383W (C383W in the GO-N6M1 background) | Fusarium graminearum | D-galactose | 0.011 | 60 | 1.9 × 10⁻⁴ | pH 7, 25 °C | Open record: galactose oxidase variant S10P/M70V/G195E/V494A/N535D/C383W (C383W in the GO-N6M1 background), D-galactose |