Values CatLog read from this paper
Substrate and metal ion promiscuity in mannosylglycerate synthase
PMID 21288903doi:10.1074/jbc.m110.199844Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 42 records (80 values) from this paper: mannosylglycerate synthase (MGS) from Rhodothermus marinus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 38 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mannosylglycerate synthase (MGS) | Rhodothermus marinus | D-glycerateGDP-mannose fixed at 500 µM; Table 2 | 0.032 | 0.0022 | 14 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS), D-glycerate |
| mannosylglycerate synthase (MGS) | Rhodothermus marinus | D-glyceratebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | 1.92 | 0.0039 | 490 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS), D-glycerate |
| mannosylglycerate synthase (MGS) | Rhodothermus marinus | D-glyceratebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | 0.033 | — | — | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS), D-glycerate |
| mannosylglycerate synthase (MGS) | Rhodothermus marinus | D-glycerate | 0.6 | 0.166 | 3.6 calc. | Open record: mannosylglycerate synthase (MGS), D-glycerate | |
| mannosylglycerate synthase (MGS) | Rhodothermus marinus | D-lactate | 0.017 | 0.72 | 0.023 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS), D-lactate |
| mannosylglycerate synthase (MGS) | Rhodothermus marinus | GDP-mannoseD-glycerate fixed at 100 µM | 0.018 | 0.0031 | 5.9 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS), GDP-mannose |
| mannosylglycerate synthase (MGS) | Rhodothermus marinus | GDP-mannosebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | — | 0.059 | — | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS), GDP-mannose |
| mannosylglycerate synthase (MGS)variant D135A | Rhodothermus marinus | D-glycerate | 0.065 | 13 | 0.0050 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant D135A, D-glycerate |
| mannosylglycerate synthase (MGS)variant D135A | Rhodothermus marinus | GDP-mannose | 0.045 | 0.0063 | 7.1 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant D135A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant E166A | Rhodothermus marinus | D-glycerate | 0.023 | 0.032 | 0.73 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant E166A, D-glycerate |
| mannosylglycerate synthase (MGS)variant E166A | Rhodothermus marinus | GDP-mannose | 0.028 | 0.0096 | 3.0 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant E166A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant H217A | Rhodothermus marinus | D-glycerateGDP-mannose fixed at 500 µM; Table 2 | 0.27 | 1.9 | 0.14 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant H217A, D-glycerate |
| mannosylglycerate synthase (MGS)variant H217A | Rhodothermus marinus | D-glyceratebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | 0.083 | 1.322 | 0.063 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant H217A, D-glycerate |
| mannosylglycerate synthase (MGS)variant H217A | Rhodothermus marinus | D-glyceratebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | 0.58 | 1.61 | 0.36 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant H217A, D-glycerate |
| mannosylglycerate synthase (MGS)variant H217A | Rhodothermus marinus | GDP-mannoseD-glycerate fixed at 10 mM; Table 2 | 0.48 | 0.012 | 40 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant H217A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant H217A | Rhodothermus marinus | GDP-mannosebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | — | 0.046 | — | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant H217A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant H217A | Rhodothermus marinus | GDP-mannosebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | — | 0.014 | — | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant H217A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant K76A | Rhodothermus marinus | D-glycerate | 0.075 | 0.03 | 2.5 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant K76A, D-glycerate |
| mannosylglycerate synthase (MGS)variant K76A | Rhodothermus marinus | GDP-mannose | 0.068 | 0.011 | 6.2 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant K76A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant K9A | Rhodothermus marinus | D-glycerate | 0.115 | 0.0014 | 82 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant K9A, D-glycerate |
| mannosylglycerate synthase (MGS)variant K9A | Rhodothermus marinus | GDP-mannose | 0.082 | 0.0017 | 48 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant K9A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant M229A | Rhodothermus marinus | D-glycerate | 0.0135 | 0.0078 | 1.7 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant M229A, D-glycerate |
| mannosylglycerate synthase (MGS)variant M229A | Rhodothermus marinus | GDP-mannose | 0.127 | 0.0028 | 45 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant M229A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant Q66A | Rhodothermus marinus | D-glycerate | 2.3 | 0.07 | 33 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Q66A, D-glycerate |
| mannosylglycerate synthase (MGS)variant Q66A | Rhodothermus marinus | GDP-mannose | 0.97 | 0.1 | 9.7 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Q66A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant R218A | Rhodothermus marinus | D-glycerate | 0.25 | 0.0058 | 43 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant R218A, D-glycerate |
| mannosylglycerate synthase (MGS)variant R218A | Rhodothermus marinus | GDP-mannose | 0.18 | 0.033 | 5.6 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant R218A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant R73A | Rhodothermus marinus | D-glycerate | 0.055 | 0.0038 | 14 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant R73A, D-glycerate |
| mannosylglycerate synthase (MGS)variant R73A | Rhodothermus marinus | GDP-mannose | 0.047 | 0.001 | 47 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant R73A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant R73G | Rhodothermus marinus | D-glycerate | 0.06 | 0.0041 | 15 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant R73G, D-glycerate |
| mannosylglycerate synthase (MGS)variant R73G | Rhodothermus marinus | GDP-mannose | 0.06 | 0.0041 | 15 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant R73G, GDP-mannose |
| mannosylglycerate synthase (MGS)variant T139A | Rhodothermus marinus | D-glycerate | 0.017 | 3.3 | 0.0051 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant T139A, D-glycerate |
| mannosylglycerate synthase (MGS)variant T139A | Rhodothermus marinus | GDP-mannose | 0.0085 | 1.1 × 10⁻⁴ | 77 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant T139A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant W189A | Rhodothermus marinus | D-glycerate | 0.077 | 0.015 | 5.1 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant W189A, D-glycerate |
| mannosylglycerate synthase (MGS)variant W189A | Rhodothermus marinus | GDP-mannose | 0.067 | 0.0028 | 24 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant W189A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant Y220A | Rhodothermus marinus | D-glycerateGDP-mannose fixed at 500 µM (Table 3 footnote b) | 0.067 | 1.1 | 0.061 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Y220A, D-glycerate |
| mannosylglycerate synthase (MGS)variant Y220A | Rhodothermus marinus | D-glyceratebi-substrate kinetics: both substrates varied at 1/2; 1; 2 | 0.067 | 1.99 | 0.034 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Y220A, D-glycerate |
| mannosylglycerate synthase (MGS)variant Y220A | Rhodothermus marinus | GDP-mannose | 0.042 | 0.0029 | 14 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Y220A, GDP-mannose |
| mannosylglycerate synthase (MGS)variant Y220F | Rhodothermus marinus | D-glycerate | 0.07 | 3.8 | 0.018 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Y220F, D-glycerate |
| mannosylglycerate synthase (MGS)variant Y220F | Rhodothermus marinus | GDP-mannose | 0.037 | 0.0065 | 5.6 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Y220F, GDP-mannose |
| mannosylglycerate synthase (MGS)variant Y37A | Rhodothermus marinus | D-glycerate | 0.18 | 0.017 | 11 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Y37A, D-glycerate |
| mannosylglycerate synthase (MGS)variant Y37A | Rhodothermus marinus | GDP-mannose | 0.163 | 0.0027 | 60 calc. | pH 7, 23 °C | Open record: mannosylglycerate synthase (MGS) variant Y37A, GDP-mannose |