Values CatLog read from this paper
Swapping FAD binding motifs between plastidic and bacterial ferredoxin-NADP(H) reductases
PMID 21306142doi:10.1021/bi101772aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 28 records (78 values) from this paper: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR), plastidic ferredoxin-NADP(H) reductase (pea FNR) from Escherichia coli, Pisum sativum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) | Escherichia coli | cytochrome c | 2.12 | — | — | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR), cytochrome c |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) | Escherichia coli | ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin | 12.3 | 7.4 × 10⁻⁵ | 1.649 × 10⁵ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR), ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) | Escherichia coli | ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin | 22.8 | 0.0014 | 1.63 × 10⁴ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR), ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) | Escherichia coli | flavodoxin A from E. coli (Fld), FMN-containing | 8.9 | 0.0023 | 3.9 × 10³ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR), flavodoxin A from E. coli (Fld), FMN-containing |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) | Escherichia coli | NADPH | 38.2 | 0.0083 | 4.6 × 10³ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR), NADPH |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted) | Escherichia coli | cytochrome c | 4.46 | — | — | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted), cytochrome c |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted) | Escherichia coli | ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin | 1.2 | 2.4 × 10⁻⁵ | 5 × 10⁴ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted), ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted) | Escherichia coli | ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin | 3.7 | 0.0076 | 500 | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted), ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted) | Escherichia coli | NADH | 45.3 | 3 | 15 | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted), NADH |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted) | Escherichia coli | NADPH | 99 | 0.003 | 3.3 × 10⁴ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) iloopΔW248 (pea β-hairpin inserted and C-terminal Trp248 deleted), NADPH |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin) | Escherichia coli | ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin | 12.8 | 9.5 × 10⁻⁵ | 1.338 × 10⁵ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin), ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin) | Escherichia coli | ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin | 19.4 | 0.0018 | 1.07 × 10⁴ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin), ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin) | Escherichia coli | flavodoxin A from E. coli (Fld), FMN-containing | 7.5 | 0.0026 | 2.9 × 10³ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin), flavodoxin A from E. coli (Fld), FMN-containing |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin) | Escherichia coli | NADPH | 24.5 | 0.0036 | 6.8 × 10³ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) variant iloop (Pro71-Asp72 replaced by pea Val113-Lys123 β-hairpin), NADPH |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)ΔW248 (C-terminal Trp248 deleted) | Escherichia coli | cytochrome c | 5.06 | — | — | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) ΔW248 (C-terminal Trp248 deleted), cytochrome c |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)ΔW248 (C-terminal Trp248 deleted) | Escherichia coli | ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin | 1.4 | 7 × 10⁻⁵ | 1.99 × 10⁴ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) ΔW248 (C-terminal Trp248 deleted), ferredoxin from E. coli (Fdx), [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)ΔW248 (C-terminal Trp248 deleted) | Escherichia coli | ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin | 4.6 | 0.0062 | 740 | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) ΔW248 (C-terminal Trp248 deleted), ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)ΔW248 (C-terminal Trp248 deleted) | Escherichia coli | NADH | 54.1 | 6.5 | 8 | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) ΔW248 (C-terminal Trp248 deleted), NADH |
| bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR)ΔW248 (C-terminal Trp248 deleted) | Escherichia coli | NADPH | 95.5 | 0.0024 | 3.98 × 10⁴ | pH 8, 30 °C | Open record: bacterial ferredoxin-NADP(H) reductase (E. coli FNR, FPR) ΔW248 (C-terminal Trp248 deleted), NADPH |
| plastidic ferredoxin-NADP(H) reductase (pea FNR) | Pisum sativum | ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin | 75 | 0.0022 | 3.41 × 10⁴ | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR), ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin |
| plastidic ferredoxin-NADP(H) reductase (pea FNR) | Pisum sativum | NADH | 7 | 14.3 | 0.5 | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR), NADH |
| plastidic ferredoxin-NADP(H) reductase (pea FNR) | Pisum sativum | NADPH | 374.3 | 0.0153 | 2.45 × 10⁴ | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR), NADPH |
| plastidic ferredoxin-NADP(H) reductase (pea FNR)variant W309 (extra C-terminal Trp added) | Pisum sativum | ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin | 35.9 | 9 × 10⁻⁴ | 3.98 × 10⁴ | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR) variant W309 (extra C-terminal Trp added), ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin |
| plastidic ferredoxin-NADP(H) reductase (pea FNR)variant W309 (extra C-terminal Trp added) | Pisum sativum | NADH | 5 | 5.9 | 0.8 | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR) variant W309 (extra C-terminal Trp added), NADH |
| plastidic ferredoxin-NADP(H) reductase (pea FNR)variant W309 (extra C-terminal Trp added) | Pisum sativum | NADPH | 360.5 | 0.0207 | 1.74 × 10⁴ | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR) variant W309 (extra C-terminal Trp added), NADPH |
| plastidic ferredoxin-NADP(H) reductase (pea FNR)variant ΔloopW309 (β-hairpin 112-123 replaced by bacterial Val-Pro-Asp, plus extra C-terminal Trp) | Pisum sativum | ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin | 32.7 | 9 × 10⁻⁴ | 3.63 × 10⁴ | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR) variant ΔloopW309 (β-hairpin 112-123 replaced by bacterial Val-Pro-Asp, plus extra C-terminal Trp), ferredoxin from pea (Fd), recombinant [2Fe-2S] ferredoxin |
| plastidic ferredoxin-NADP(H) reductase (pea FNR)variant ΔloopW309 (β-hairpin 112-123 replaced by bacterial Val-Pro-Asp, plus extra C-terminal Trp) | Pisum sativum | NADH | 2 | 3.7 | 0.6 | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR) variant ΔloopW309 (β-hairpin 112-123 replaced by bacterial Val-Pro-Asp, plus extra C-terminal Trp), NADH |
| plastidic ferredoxin-NADP(H) reductase (pea FNR)variant ΔloopW309 (β-hairpin 112-123 replaced by bacterial Val-Pro-Asp, plus extra C-terminal Trp) | Pisum sativum | NADPH | 251.1 | 0.0147 | 1.71 × 10⁴ | pH 8, 30 °C | Open record: plastidic ferredoxin-NADP(H) reductase (pea FNR) variant ΔloopW309 (β-hairpin 112-123 replaced by bacterial Val-Pro-Asp, plus extra C-terminal Trp), NADPH |