Values CatLog read from this paper
Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase
PMID 2139796doi:10.1021/bi00458a013Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (17 values) from this paper: isochorismatase (EntB) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isochorismatase (EntB) | Escherichia coli | (±)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid | 5.2 | 0.023 | 220 | pH 7.01, 37 °C | Open record: isochorismatase (EntB), (±)-3-[(1-carboxyethenyl)oxy]-2-hydroxy-6-cyclohexene-1-carboxylic acid |
| isochorismatase (EntB) | Escherichia coli | (±)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid | 22 | 0.086 | 250 | pH 7.01, 37 °C | Open record: isochorismatase (EntB), (±)-3-[(carboxyethenyl)oxy]-1-cyclohexene-1-carboxylic acid |
| isochorismatase (EntB) | Escherichia coli | (±)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid | 16.3 | 0.28 | 58 | pH 7.01, 37 °C | Open record: isochorismatase (EntB), (±)-cis-3-[(carboxyethenyl)oxy]-4-cyclohexene-1-carboxylic acid |
| isochorismatase (EntB) | Escherichia coli | (±)-disodium 3-[(1-carboxylatoethenyl)oxy]cyclohepta-1,6-diene-1-carboxylate | 9 | 0.12 | 75 | pH 7.01, 37 °C | Open record: isochorismatase (EntB), (±)-disodium 3-[(1-carboxylatoethenyl)oxy]cyclohepta-1,6-diene-1-carboxylate |
| isochorismatase (EntB) | Escherichia coli | isochorismate (1) | 10 | 0.015 | 670 | pH 7.01, 37 °C | Open record: isochorismatase (EntB), isochorismate (1) |
| isochorismatase (EntB) | Escherichia coli | synthetic (±)-isochorismate (1) | 8.7 | 0.0106 | 820 calc. | pH 7.01, 37 °C | Open record: isochorismatase (EntB), synthetic (±)-isochorismate (1) |