Values CatLog read from this paper
Gentisate 1,2-dioxygenase from pseudomonas. Purification, characterization, and comparison of the enzymes from Pseudomonas testosteroni and Pseudomonas acidovorans
PMID 2156846doi:10.1016/s0021-9258(19)39326-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 29 records (55 values) from this paper: gentisate 1,2-dioxygenase from Pseudomonas acidovorans, Pseudomonas testosteroni.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 1,4-dihydroxy-2-naphthoate | — | 0.045 | 180 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 1,4-dihydroxy-2-naphthoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxy-3,4-dimethylbenzoate | — | 0.14 | 180 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-3,4-dimethylbenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxy-3-isopropylbenzoate | — | 0.048 | 1.1 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-3-isopropylbenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxy-3-methoxybenzoate | — | 0.025 | 2.5 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-3-methoxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxy-3-methylbenzoate | — | 0.027 | 5.1 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-3-methylbenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxy-4-methoxybenzoate | — | 0.035 | 370 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-4-methoxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxy-4-methylbenzoate | — | 0.057 | 340 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-4-methylbenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxybenzoateatmospheric O2 (~260 µM) | 322 | 0.074 | 4.3 × 10³ calc. | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 2,5-dihydroxybenzoate | — | — | 4.35 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 3-bromo-2,5-dihydroxybenzoate | — | 0.018 | 4.7 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 3-bromo-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 3-ethyl-2,5-dihydroxybenzoate | — | 0.018 | 5.5 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 3-ethyl-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 3-fluoro-2,5-dihydroxybenzoate | — | 0.035 | 3 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 3-fluoro-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 4-chloro-2,5-dihydroxybenzoate | — | 0.025 | 520 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 4-chloro-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | 4-fluoro-2,5-dihydroxybenzoate | — | 0.015 | 2.8 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 4-fluoro-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas acidovorans | dioxygen | — | 0.055 | — | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, dioxygen |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 1,4-dihydroxy-2-naphthoate | — | 0.29 | 4 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 1,4-dihydroxy-2-naphthoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 2,5-dihydroxy-3-isopropylbenzoate | — | 2.75 | 0.5 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-3-isopropylbenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 2,5-dihydroxy-3-methoxybenzoate | — | 1.18 | 34 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-3-methoxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 2,5-dihydroxy-3-methylbenzoate | — | 1.75 | 120 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-3-methylbenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 2,5-dihydroxy-4-methoxybenzoate | — | 0.45 | 120 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-4-methoxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 2,5-dihydroxy-4-methylbenzoate | — | 0.52 | 22 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxy-4-methylbenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 2,5-dihydroxybenzoateatmospheric O2 (~260 µM) | 370 | 0.085 | 4.3 × 10³ calc. | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 2,5-dihydroxybenzoate | — | — | 4.3 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 3-bromo-2,5-dihydroxybenzoate | — | 0.33 | 280 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 3-bromo-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 3-ethyl-2,5-dihydroxybenzoate | — | 3.65 | 7 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 3-ethyl-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 3-fluoro-2,5-dihydroxybenzoate | — | 0.05 | 67 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 3-fluoro-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 4-chloro-2,5-dihydroxybenzoate | — | 0.24 | 150 | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 4-chloro-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | 4-fluoro-2,5-dihydroxybenzoate | — | 0.044 | 4 × 10³ | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, 4-fluoro-2,5-dihydroxybenzoate |
| gentisate 1,2-dioxygenase | Pseudomonas testosteroni | dioxygen | 642 | 0.096 | 6.7 × 10³ calc. | pH 7.4, 23 °C | Open record: gentisate 1,2-dioxygenase, dioxygen |