CatLogenzyme kinetics catalog

Values CatLog read from this paper

Binding of ATP at the active site of human pancreatic glucokinase--nucleotide-induced conformational changes with possible implications for its kinetic cooperativity

PMID 21569204doi:10.1111/j.1742-4658.2011.08160.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (8 values) from this paper: glucokinase (hexokinase IV), pancreatic isoform 1 from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 2.7.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
glucokinase (hexokinase IV), pancreatic isoform 1Homo sapiensalpha-D-glucoseGlc varied (0-60 mM) at 5 mM ATP (2.5 mM excess Mg2+)67.6——37 °COpen
glucokinase (hexokinase IV), pancreatic isoform 1Homo sapiensalpha-D-glucoseGlc varied at 0.05 mM MgATP; (0.05 mM ATP)27.8——37 °COpen
glucokinase (hexokinase IV), pancreatic isoform 1Homo sapiensMgATP^{2−}(0-3 mM, 2.5 mM excess Mg2+) at 60; cosubstrate Glc (60 mM)68.40.16430 calc.37 °COpen
glucokinase (hexokinase IV), pancreatic isoform 1Homo sapiensMgATP^{2−}(2.5 mM excess Mg2+) at 0.5; cosubstrate Glc (0.5 mM)0.7——37 °COpen
glucokinase (hexokinase IV), pancreatic isoform 1variant L146RHomo sapiensalpha-D-glucose0.77——37 °COpen
glucokinase (hexokinase IV), pancreatic isoform 1variant L146RHomo sapiensMgATP^{2−}0.61——37 °COpen
glucokinase (hexokinase IV), pancreatic isoform 1variant T228MHomo sapiensalpha-D-glucose0.007——37 °COpen