CatLogenzyme kinetics catalog

Values CatLog read from this paper

Oligopeptidase B from Serratia proteamaculans. II. Enzymatic characteristics: substrate analysis, influence of calcium ions, pH and temperature dependences

PMID 21585325doi:10.1134/s0006297911040122Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 29 records (77 values) from this paper: oligopeptidase B (PSP) from Serratia proteamaculans.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.21.83

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
oligopeptidase B (PSP)Serratia proteamaculansacetyl-Leu-Leu-Arg-p-nitroanilide50 mM CaCl2 (buffer B)16.80.55930pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansacetyl-Leu-Leu-Arg-p-nitroanilideno CaCl2 (buffer A)7.620.22434pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansacetyl-Leu-Leu-Arg-p-nitroanilide50 mM CaCl2 (buffer B)13.90.45730.5pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansacetyl-Leu-Lys-Arg-p-nitroanilideno CaCl2 (buffer A)24.30.02261.08 × 10³pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansacetyl-Leu-Lys-Arg-p-nitroanilide50 mM CaCl2 (buffer B)320.265120pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansacetyl-Leu-Lys-Arg-p-nitroanilideno CaCl2 (buffer A)23.80.01961.22 × 10³pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansbenzoyl-Pro-Phe-Arg-p-nitroanilide50 mM CaCl2 (buffer B)18.20.062293pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansbenzoyl-Pro-Phe-Arg-p-nitroanilideno CaCl2 (buffer A)2020.54377pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansbenzoyl-Pro-Phe-Arg-p-nitroanilide50 mM CaCl2 (buffer B)21.20.084251.2pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansbenzyloxycarbonyl-Ala-Ala-Arg-p-nitroanilide——13.2pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansbenzyloxycarbonyl-Phe-Arg-p-nitroanilideno CaCl2 (buffer A)280.058485pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansbenzyloxycarbonyl-Phe-Arg-p-nitroanilide50 mM CaCl2 (buffer B)35.20.0513687pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansbenzyloxycarbonyl-Phe-Arg-p-nitroanilide50 mM CaCl2 (buffer B)57.70.0732787pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzoyl-DL-arginine p-nitroanilideno CaCl2 (buffer A); 2% DMSO14.50.09160.8pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzoyl-DL-arginine p-nitroanilide50 mM CaCl2 (buffer B); 2% DMSO37.70.302125pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzoyl-DL-arginine p-nitroanilide50 mM CaCl2 (buffer B); 2% DMSO36.80.309120pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzoyl-DL-arginine p-nitroanilide50 mM CaCl2 (buffer B); 0.2-2% DMSO37.20.3125pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzoyl-DL-arginine p-nitroanilideno CaCl2 (buffer A); 10% DMSO16.080.145110.8pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzoyl-DL-arginine p-nitroanilide50 mM CaCl2 (buffer B); 10% DMSO30.20.66345pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzyloxycarbonyl-L-lysine thiobenzyl esterno CaCl2 (buffer A); Michaelis-Menten nonlinear regression55.50.096580pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzyloxycarbonyl-L-lysine thiobenzyl ester50 mM CaCl2 (buffer B)——151.5pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculansNα-benzyloxycarbonyl-L-lysine thiobenzyl ester50 mM CaCl2 (buffer B)53.80.322173pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculanssuccinyl-Ala-Ala-Pro-Arg-p-nitroanilide50 mM CaCl2 (buffer B)1.20.3233.7pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculanssuccinyl-Ala-Ala-Pro-Arg-p-nitroanilideno CaCl2 (buffer A)0.51.050.48pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculanssuccinyl-Ala-Ala-Pro-Arg-p-nitroanilide50 mM CaCl2 (buffer B)1.030.3063.42pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculanssuccinyl-Ala-Ala-Pro-Lys-p-nitroanilide50 mM CaCl2 (buffer B)——0.3pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculanssuccinyl-Ala-Ala-Pro-Lys-p-nitroanilideno CaCl2 (buffer A)——0.067pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculanssuccinyl-Ala-Ala-Pro-Lys-p-nitroanilide50 mM CaCl2 (buffer B)0.932.20.425pH 8, 25 °COpen
oligopeptidase B (PSP)Serratia proteamaculanssuccinyl-Gly-Pro-Lys-p-nitroanilide——1.12pH 8, 25 °COpen