Values CatLog read from this paper
Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: creation and characterization of highly in vivo soluble mutants
PMID 21729945doi:10.1093/protein/gzr030Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (33 values) from this paper: hydroxynitrile lyase (MeHNL) from Manihot esculenta.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| hydroxynitrile lyase (MeHNL) | Manihot esculenta | benzaldehyde | 96.6 | 23 | 4.2 | pH 5, 22 °C | Open record: hydroxynitrile lyase (MeHNL), benzaldehyde |
| hydroxynitrile lyase (MeHNL) | Manihot esculenta | mandelonitrile | 34.5 | 5.17 | 6.67 | 22 °C | Open record: hydroxynitrile lyase (MeHNL), mandelonitrile |
| hydroxynitrile lyase (MeHNL)variant H103L | Manihot esculenta | benzaldehyde | 90.4 | 13.4 | 6.75 | pH 5, 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant H103L, benzaldehyde |
| hydroxynitrile lyase (MeHNL)variant H103L | Manihot esculenta | mandelonitrile | 21.5 | 2.55 | 8.43 | 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant H103L, mandelonitrile |
| hydroxynitrile lyase (MeHNL)variant H103M | Manihot esculenta | benzaldehyde | 96.1 | 27.9 | 3.44 | pH 5, 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant H103M, benzaldehyde |
| hydroxynitrile lyase (MeHNL)variant H103M | Manihot esculenta | mandelonitrile | 23.9 | 1.86 | 12.8 | 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant H103M, mandelonitrile |
| hydroxynitrile lyase (MeHNL)variant H103Y | Manihot esculenta | benzaldehyde | 47.4 | 12.3 | 3.85 | pH 5, 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant H103Y, benzaldehyde |
| hydroxynitrile lyase (MeHNL)variant K176P | Manihot esculenta | benzaldehyde | 83.2 | 21.6 | 3.85 | pH 5, 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant K176P, benzaldehyde |
| hydroxynitrile lyase (MeHNL)variant K176P | Manihot esculenta | mandelonitrile | 41.8 | 6.98 | 6 | 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant K176P, mandelonitrile |
| hydroxynitrile lyase (MeHNL)variant K176P/K199P/K224P (MeHNL-3 Lys-Pro) | Manihot esculenta | benzaldehyde | 93.5 | 27 | 3.46 | pH 5, 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant K176P/K199P/K224P (MeHNL-3 Lys-Pro), benzaldehyde |
| hydroxynitrile lyase (MeHNL)variant K176P/K199P/K224P (MeHNL-3 Lys-Pro) | Manihot esculenta | mandelonitrile | 39.7 | 5.54 | 7.2 | 22 °C | Open record: hydroxynitrile lyase (MeHNL) variant K176P/K199P/K224P (MeHNL-3 Lys-Pro), mandelonitrile |