Values CatLog read from this paper
Switch of substrate specificity of hyperthermophilic acylaminoacyl peptidase by combination of protein and solvent engineering
PMID 21748600doi:10.1007/s13238-011-1057-7Open in Browse
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CatLog read 13 records (39 values) from this paper: acylaminoacyl peptidase (apAAP) from Aeropyrum pernix.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acylaminoacyl peptidase (apAAP) | Aeropyrum pernix | p-nitrophenyl laurate | 1.35 | 0.00862 | 157.2 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP), p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant F488A/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 12.08 | 0.00737 | 1638 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant F488A/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant F488G/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 17.17 | 0.00834 | 2059 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant F488G/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant F488P/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 8.17 | 0.00388 | 2107 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant F488P/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant F488S/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 10.45 | 0.00579 | 1804 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant F488S/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant F488W/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 1.73 | 0.00101 | 1712 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant F488W/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant F488Y/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 2.05 | 7.6 × 10⁻⁴ | 2703 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant F488Y/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant R526V | Aeropyrum pernix | p-nitrophenyl laurate | 5.47 | 0.0118 | 462.5 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant R526V, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 7.96 | 0.00122 | 6528 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant W474A/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 12.61 | 0.00183 | 6876 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant W474A/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant W474Q/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 5.29 | 7.5 × 10⁻⁴ | 7018 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant W474Q/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant W474V/F488G/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 26.25 | 0.00234 | 11,218 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant W474V/F488G/R526V/T560W, p-nitrophenyl laurate |
| acylaminoacyl peptidase (apAAP)variant W474V/R526V/T560W | Aeropyrum pernix | p-nitrophenyl laurate | 23.5 | 0.00243 | 9662 | pH 8, 60 °C | Open record: acylaminoacyl peptidase (apAAP) variant W474V/R526V/T560W, p-nitrophenyl laurate |