Values CatLog read from this paper
Evidence for the mechanism of hydroxylation by 4-hydroxyphenylpyruvate dioxygenase and hydroxymandelate synthase from intermediate partitioning in active site variants
PMID 21815644doi:10.1021/bi2009344Open in Browse
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CatLog read 9 records (27 values) from this paper: 4-hydroxyphenylpyruvate dioxygenase (HPPD), hydroxymandelate synthase (HMS) from Streptomyces avermitilis, Amycolatopsis orientalis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-hydroxyphenylpyruvate dioxygenase (HPPD) | Streptomyces avermitilis | 4-hydroxyphenylpyruvate | 6.8 | 0.027 | 254 | pH 7, 25 °C | Open record: 4-hydroxyphenylpyruvate dioxygenase (HPPD), 4-hydroxyphenylpyruvate |
| 4-hydroxyphenylpyruvate dioxygenase (HPPD)variant N245I | Streptomyces avermitilis | 4-hydroxyphenylpyruvate | 0.64 | 0.027 | 24 | pH 7, 25 °C | Open record: 4-hydroxyphenylpyruvate dioxygenase (HPPD) variant N245I, 4-hydroxyphenylpyruvate |
| 4-hydroxyphenylpyruvate dioxygenase (HPPD)variant N245Q | Streptomyces avermitilis | 4-hydroxyphenylpyruvate | 0.03 | 0.015 | 2.4 | pH 7, 25 °C | Open record: 4-hydroxyphenylpyruvate dioxygenase (HPPD) variant N245Q, 4-hydroxyphenylpyruvate |
| 4-hydroxyphenylpyruvate dioxygenase (HPPD)variant P243T | Streptomyces avermitilis | 4-hydroxyphenylpyruvate | 2.1 | 0.0087 | 241 | pH 7, 25 °C | Open record: 4-hydroxyphenylpyruvate dioxygenase (HPPD) variant P243T, 4-hydroxyphenylpyruvate |
| 4-hydroxyphenylpyruvate dioxygenase (HPPD)variant S230A | Streptomyces avermitilis | 4-hydroxyphenylpyruvate | 2.8 | 0.047 | 60 | pH 7, 25 °C | Open record: 4-hydroxyphenylpyruvate dioxygenase (HPPD) variant S230A, 4-hydroxyphenylpyruvate |
| hydroxymandelate synthase (HMS) | Amycolatopsis orientalis | 4-hydroxyphenylpyruvate | 3.7 | 0.0476 | 77 | pH 7, 25 °C | Open record: hydroxymandelate synthase (HMS), 4-hydroxyphenylpyruvate |
| hydroxymandelate synthase (HMS)variant I216N | Amycolatopsis orientalis | 4-hydroxyphenylpyruvate | 1.4 | 0.0143 | 98.5 | pH 7, 25 °C | Open record: hydroxymandelate synthase (HMS) variant I216N, 4-hydroxyphenylpyruvate |
| hydroxymandelate synthase (HMS)variant S201A | Amycolatopsis orientalis | 4-hydroxyphenylpyruvate | 3 | 0.028 | 108 | pH 7, 25 °C | Open record: hydroxymandelate synthase (HMS) variant S201A, 4-hydroxyphenylpyruvate |
| hydroxymandelate synthase (HMS)variant T214P | Amycolatopsis orientalis | 4-hydroxyphenylpyruvate | 1.18 | 0.0205 | 57.5 | pH 7, 25 °C | Open record: hydroxymandelate synthase (HMS) variant T214P, 4-hydroxyphenylpyruvate |