Values CatLog read from this paper
Subcloning, characterization, and affinity labeling of Escherichia coli glycinamide ribonucleotide transformylase
PMID 2185839doi:10.1021/bi00458a014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (27 values) from this paper: glycinamide ribonucleotide transformylase (GAR TFase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | (6R)-N10-formyltetrahydrofolate | 13.5 | 0.0848 | 160 | pH 7.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), (6R)-N10-formyltetrahydrofolate |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | (6R)-N10-formyltetrahydrofolate | 20.7 | 0.0775 | 270 | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), (6R)-N10-formyltetrahydrofolate |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | beta-glycinamide ribonucleotideDDF formation followed at 295 nm; cosubstrate N10-formyl-DDF | — | 0.0192 | — | pH 7.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), beta-glycinamide ribonucleotide |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | beta-glycinamide ribonucleotideDDF formation followed at 295 nm; cosubstrate N10-formyl-DDF | — | 0.0235 | — | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), beta-glycinamide ribonucleotide |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | beta-glycinamide ribonucleotideargon-saturated buffer with 10 mM beta-mercaptoethanol | — | 0.0122 | — | pH 7.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), beta-glycinamide ribonucleotide |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | beta-glycinamide ribonucleotideargon-saturated buffer with 10 mM beta-mercaptoethanol | — | 0.0081 | — | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), beta-glycinamide ribonucleotide |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | carbocyclic glycinamide ribonucleotide | 33 | 0.0465 | 710 calc. | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), carbocyclic glycinamide ribonucleotide |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | N10-formyl-5,8-dideazafolate | 16.1 | 0.0167 | 960 | pH 7.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), N10-formyl-5,8-dideazafolate |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | N10-formyl-5,8-dideazafolate | 40 | 0.0365 | 1.1 × 10³ | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), N10-formyl-5,8-dideazafolate |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | N10-formyl-5-deazafolate | 1.52 | 0.19 | 8 | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), N10-formyl-5-deazafolate |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | N10-formyl-8-deazafolate | 0.069 | 0.15 | 0.5 | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), N10-formyl-8-deazafolate |
| glycinamide ribonucleotide transformylase (GAR TFase) | Escherichia coli | N10-formylfolic acid | 0.016 | 0.6 | 0.02 | pH 8.5, 26 °C | Open record: glycinamide ribonucleotide transformylase (GAR TFase), N10-formylfolic acid |