Values CatLog read from this paper
An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD-Dependent Glutamate Dehydrogenase
PMID 21876794doi:10.4061/2011/595793Open in Browse
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CatLog read 36 records (106 values) from this paper: NAD+-dependent glutamate dehydrogenase (CsGDH) from Clostridium symbiosum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NAD+-dependent glutamate dehydrogenase (CsGDH) | Clostridium symbiosum | NADH | 19.9 | 0.036 | 553 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH), NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH) | Clostridium symbiosum | NADH | 130.2 | 0.02 | 6.5 × 10³ | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH), NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH) | Clostridium symbiosum | NADH | 232 | 0.078 | 2974 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH), NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH) | Clostridium symbiosum | NADPH | 3.59 | 0.061 | 58.9 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH), NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH) | Clostridium symbiosum | NADPH | 2.87 | 0.09 | 31.9 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH), NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH) | Clostridium symbiosum | NADPH | 0.82 | 0.53 | 1.54 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH), NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant A242G | Clostridium symbiosum | NADH | 11.2 | 0.034 | 329 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant A242G, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant A242G | Clostridium symbiosum | NADH | 65.1 | 0.025 | 2604 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant A242G, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant A242G | Clostridium symbiosum | NADH | 177 | 0.085 | 2082 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant A242G, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant A242G | Clostridium symbiosum | NADPH | 1.43 | 0.074 | 19.3 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant A242G, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant A242G | Clostridium symbiosum | NADPH | 1.87 | 0.386 | 4.84 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant A242G, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant A242G | Clostridium symbiosum | NADPH | 0.15 | — | — | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant A242G, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant D263K | Clostridium symbiosum | NADH | 14.6 | 0.022 | 663 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant D263K, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant D263K | Clostridium symbiosum | NADH | 80 | 0.023 | 3478 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant D263K, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant D263K | Clostridium symbiosum | NADH | 150 | 0.091 | 1648 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant D263K, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant D263K | Clostridium symbiosum | NADPH | 20 | 0.068 | 294 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant D263K, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant D263K | Clostridium symbiosum | NADPH | 26.3 | 0.107 | 246 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant D263K, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant D263K | Clostridium symbiosum | NADPH | 37.5 | 0.154 | 232 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant D263K, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S | Clostridium symbiosum | NADH | 13.6 | 0.028 | 485 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S | Clostridium symbiosum | NADH | 139 | 0.04 | 3475 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S | Clostridium symbiosum | NADH | 402 | 0.23 | 1747 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S | Clostridium symbiosum | NADPH | 5 | 1 | 5 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S | Clostridium symbiosum | NADPH | 11.1 | 0.23 | 48.3 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S | Clostridium symbiosum | NADPH | 3.61 | 0.462 | 7.81 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S/P262S | Clostridium symbiosum | NADH | 13.9 | 0.048 | 289 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S/P262S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S/P262S | Clostridium symbiosum | NADH | 105 | 0.035 | 3 × 10³ | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S/P262S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S/P262S | Clostridium symbiosum | NADH | 133 | 0.204 | 665 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S/P262S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S/P262S | Clostridium symbiosum | NADPH | 11.2 | 2 | 5.75 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S/P262S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S/P262S | Clostridium symbiosum | NADPH | 83.3 | 0.33 | 252 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S/P262S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant F238S/P262S | Clostridium symbiosum | NADPH | 142 | 0.56 | 221 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant F238S/P262S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant P262S | Clostridium symbiosum | NADH | 14.4 | 0.018 | 800 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant P262S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant P262S | Clostridium symbiosum | NADH | 160 | 0.047 | 3404 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant P262S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant P262S | Clostridium symbiosum | NADH | 490 | 0.142 | 3.45 × 10³ | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant P262S, NADH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant P262S | Clostridium symbiosum | NADPH | 14.8 | 1.1 | 13.5 | pH 6, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant P262S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant P262S | Clostridium symbiosum | NADPH | 62.5 | 0.11 | 568 | pH 7, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant P262S, NADPH |
| NAD+-dependent glutamate dehydrogenase (CsGDH)variant P262S | Clostridium symbiosum | NADPH | 88.5 | 0.33 | 268 | pH 8, 25 °C | Open record: NAD+-dependent glutamate dehydrogenase (CsGDH) variant P262S, NADPH |