Values CatLog read from this paper
Redefining the minimal substrate tolerance of mandelate racemase. Racemization of trifluorolactate
PMID 21894901doi:10.1021/bi201188jOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (22 values) from this paper: mandelate racemase from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mandelate racemase | Pseudomonas putida | (R)-3,3,3-trifluoro-2-hydroxypropanoate | 2 | 1.2 | 1.6 | pH 7.5, 25 °C | Open record: mandelate racemase, (R)-3,3,3-trifluoro-2-hydroxypropanoate |
| mandelate racemase | Pseudomonas putida | (R)-mandelate | 792 | 1.2 | 650 | pH 7.5, 25 °C | Open record: mandelate racemase, (R)-mandelate |
| mandelate racemase | Pseudomonas putida | (S)-3,3,3-trifluoro-2-hydroxypropanoate | 2.5 | 1.74 | 1.4 | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-3,3,3-trifluoro-2-hydroxypropanoate |
| mandelate racemase | Pseudomonas putida | (S)-3,3,3-trifluoro-2-hydroxypropanoate10.0% sucrose as viscosogen (η/ηo = 1.32) | 2.21 | 1.71 | 1.29 calc. | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-3,3,3-trifluoro-2-hydroxypropanoate |
| mandelate racemase | Pseudomonas putida | (S)-3,3,3-trifluoro-2-hydroxypropanoate20.0% sucrose as viscosogen (η/ηo = 1.88) | 2.53 | 1.64 | 1.54 calc. | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-3,3,3-trifluoro-2-hydroxypropanoate |
| mandelate racemase | Pseudomonas putida | (S)-3,3,3-trifluoro-2-hydroxypropanoate27.5% sucrose as viscosogen (η/ηo = 2.48) | 2.61 | 1.49 | 1.75 calc. | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-3,3,3-trifluoro-2-hydroxypropanoate |
| mandelate racemase | Pseudomonas putida | (S)-3,3,3-trifluoro-2-hydroxypropanoate32.5% sucrose as viscosogen (η/ηo = 3.06) | 2.4 | 1.53 | 1.6 calc. | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-3,3,3-trifluoro-2-hydroxypropanoate |
| mandelate racemase | Pseudomonas putida | (S)-3,3,3-trifluoro-2-hydroxypropanoate35.0% sucrose as viscosogen (η/ηo = 3.42) | 2.04 | 1.42 | 1.44 calc. | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-3,3,3-trifluoro-2-hydroxypropanoate |
| mandelate racemase | Pseudomonas putida | (S)-mandelate | 637 | 1 | 620 | pH 7.5, 25 °C | Open record: mandelate racemase, (S)-mandelate |