CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structure-based alteration of substrate specificity and catalytic activity of sulfite oxidase from sulfite oxidation to nitrate reduction

PMID 22263579doi:10.1021/bi201206vOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 127 records (161 values) from this paper: sulfite oxidase from Homo sapiens, Gallus gallus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.8.3.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
sulfite oxidaseGallus gallussulfite71.40.008538.3 × 10³pH 8.5, 25 °COpen
sulfite oxidaseGallus gallussulfite36.10.001332.7 × 10⁴pH 7, 25 °COpen
sulfite oxidasevariant Y322N/R450MGallus gallusnitrate0.7511.1340.66pH 8.5, 25 °COpen
sulfite oxidasevariant Y322N/R450MGallus gallusnitrate0.650.4451.4pH 7, 25 °COpen
sulfite oxidasevariant Y322N/R450MGallus gallussulfite2.5211.730.21pH 8.5, 25 °COpen
sulfite oxidasevariant Y322N/R450MGallus gallussulfite4.830.5618.6pH 7, 25 °COpen
sulfite oxidasevariant Y322N/R450M/V452MGallus gallusnitrate0.3770.15112.4pH 8.5, 25 °COpen
sulfite oxidasevariant Y322N/R450M/V452MGallus gallusnitrate0.960.072713pH 7, 25 °COpen
sulfite oxidasevariant Y322N/R450M/V452MGallus gallussulfite4.16.750.61pH 8.5, 25 °COpen
sulfite oxidasevariant Y322N/R450M/V452MGallus gallussulfite2.180.573.8pH 7, 25 °COpen
sulfite oxidaseHomo sapienscytochrome c—0.0044—25 °COpen
sulfite oxidaseHomo sapiensnitrateTable 8—43—pH 8.5, 25 °COpen
sulfite oxidaseHomo sapiensnitrateTable 90.132.270.057 calc.pH 8.5, 25 °COpen
sulfite oxidaseHomo sapiensnitrate0.061.410.043 calc.pH 7, 25 °COpen
sulfite oxidaseHomo sapienssulfiteTable 1—0.0529—pH 10, 25 °COpen
sulfite oxidaseHomo sapienssulfiteTable 213——pH 10, 25 °COpen
sulfite oxidaseHomo sapienssulfiteTable 7——246pH 10, 25 °COpen
sulfite oxidasevariant R472MHomo sapienscytochrome c—0.0041—25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 1—0.00195—pH 6, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO—0.00245—pH 7, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 1—0.00472—pH 8, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO—0.00938—pH 8.5, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 1—0.0212—pH 9, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 1—0.0537—pH 9.5, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 1—0.0969—pH 10, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 25.02——pH 6, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO3.6——pH 7, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 23.48——pH 8, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO3.77——pH 8.5, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 23.44——pH 9, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 21.81——pH 9.5, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteTable 20.46——pH 10, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteferricyanide reduction followed at 420 nm; 1.5-5.0 µg/ml SO—0.094—pH 8.5, 25 °COpen
sulfite oxidasevariant R472MHomo sapienssulfiteferricyanide reduction followed at 420 nm; 1.5-5.0 µg/ml SO—0.005—pH 7, 25 °COpen
sulfite oxidasevariant R472QHomo sapienscytochrome c—0.004—25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 1—0.00232—pH 6, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO—0.00351—pH 7, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 1—0.013—pH 8, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO—0.0162—pH 8.5, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 1—0.0453—pH 9, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 1—0.0967—pH 9.5, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 1—0.1817—pH 10, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 28.17——pH 6, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO5.77——pH 7, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 29.34——pH 8, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfitecytochrome c reduction followed at 550 nm; 0.50-2.5 µg/ml SO4.58——pH 8.5, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 24.24——pH 9, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 21.67——pH 9.5, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteTable 20.52——pH 10, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteferricyanide reduction followed at 420 nm; 1.5-5.0 µg/ml SO—0.223—pH 8.5, 25 °COpen
sulfite oxidasevariant R472QHomo sapienssulfiteferricyanide reduction followed at 420 nm; 1.5-5.0 µg/ml SO—0.0128—pH 7, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 5—4.6 × 10⁻⁴—pH 6, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 5—0.00134—pH 7, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 5—0.00319—pH 8, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 5—0.00354—pH 8.5, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 5—0.0144—pH 9, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 5—0.0319—pH 9.5, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 5—0.0402—pH 10, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 65.96——pH 6, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 611.4——pH 7, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 617.8——pH 8, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 615.8——pH 8.5, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 619.6——pH 9, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 617.1——pH 9.5, 25 °COpen
sulfite oxidasevariant V474MHomo sapienssulfiteTable 612.4——pH 10, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 5—0.0614—pH 6, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 5—0.0877—pH 7, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 5—0.2827—pH 8, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 5—0.7125—pH 8.5, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 5—3.34—pH 9, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 5—39.9—pH 9.5, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 5—59.66—pH 10, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 61.44——pH 6, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 61.73——pH 7, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 62.05——pH 8, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 62.25——pH 8.5, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 62.31——pH 9, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 64.94——pH 9.5, 25 °COpen
sulfite oxidasevariant Y343F/R472QHomo sapienssulfiteTable 62.96——pH 10, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 5—0.0953—pH 6, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 5—0.0947—pH 7, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 5—0.297—pH 8, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 5—0.85—pH 8.5, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 5—2.46—pH 9, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 5—9.37—pH 9.5, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 5—12.01—pH 10, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 63.17——pH 6, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 612.77——pH 7, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 613.75——pH 8, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 616.91——pH 8.5, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 615.54——pH 9, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 68.11——pH 9.5, 25 °COpen
sulfite oxidasevariant Y343NHomo sapienssulfiteTable 64.38——pH 10, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapiensnitrateTable 80.650.5681.1pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapiensnitrateTable 80.820.02434pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapiensnitrateTable 90.470.8720.54 calc.pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapiensnitrateTable 90.620.04215 calc.pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 5—16.8—pH 6, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 5—4.64—pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 5—19.28—pH 8, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 5—42.99—pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 5—85.64—pH 9, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 5—208—pH 9.5, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 61.35——pH 6, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 61.13——pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 61.33——pH 8, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 61.42——pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 61.13——pH 9, 25 °COpen
sulfite oxidasevariant Y343N/R472MHomo sapienssulfiteTable 60.97——pH 9.5, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapiensnitrateTable 80.670.1923.5pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapiensnitrateTable 81.070.0335pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapiensnitrateTable 90.630.3711.7 calc.pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapiensnitrateTable 91.930.03752 calc.pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 5—1.1—pH 6, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 5—1.42—pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 5—2.14—pH 8, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 5—14—pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 5—55.5—pH 9, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 5—111—pH 9.5, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 5—418—pH 10, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 61.9——pH 6, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 63.37——pH 7, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 63.58——pH 8, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 63.9——pH 8.5, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 65.6——pH 9, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 65.23——pH 9.5, 25 °COpen
sulfite oxidasevariant Y343N/R472M/V474MHomo sapienssulfiteTable 62.8——pH 10, 25 °COpen