Values CatLog read from this paper
Yeast mitochondrial threonyl-tRNA synthetase recognizes tRNA isoacceptors by distinct mechanisms and promotes CUN codon reassignment
PMID 22343532doi:10.1073/pnas.1200109109Open in Browse
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CatLog read 40 records (120 values) from this paper: mitochondrial threonyl-tRNA synthetase (MST1) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant A35G/G36U | 0.045 | 3.3 × 10⁻⁴ | 150 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant A35G/G36U |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant A73G | 0.0062 | 5 × 10⁻⁴ | 14.8 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant A73G |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant G37A | 0.048 | 0.0012 | 40 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant G37A |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant U38A | 0.022 | 0.0018 | 11.5 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant U38A |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant ΔU32 | 0.0142 | 0.0012 | 13.2 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr1 in vitro transcript, mutant ΔU32 |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant G35A/U36G | 0.00127 | 0.0031 | 0.5 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant G35A/U36G |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant G35A/U36G/A37G | 0.0017 | 9.4 × 10⁻⁴ | 1.8 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant G35A/U36G/A37G |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant G35A/U36G/A37G/A38U | 0.00105 | 0.0023 | 0.47 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant G35A/U36G/A37G/A38U |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant insU32/G35A/U36G | 9.7 × 10⁻⁴ | 0.0012 | 0.83 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant insU32/G35A/U36G |
| mitochondrial threonyl-tRNA synthetase (MST1) | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant insU32/G35A/U36G/A37G/A38U | 0.013 | 0.0016 | 8.3 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1), S. cerevisiae mitochondrial tRNAThr2 in vitro transcript, mutant insU32/G35A/U36G/A37G/A38U |
| mitochondrial threonyl-tRNA synthetase (MST1)variant D423A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.067 | 2 × 10⁻⁴ | 337 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant D423A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant D423A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.055 | 3.1 × 10⁻⁴ | 185 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant D423A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant D437A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.053 | 2.4 × 10⁻⁴ | 263 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant D437A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant D437A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.045 | 3.7 × 10⁻⁴ | 132 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant D437A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant E401A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.073 | 1.3 × 10⁻⁴ | 582 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant E401A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant E401A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.067 | 2.7 × 10⁻⁴ | 277 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant E401A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant K408A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.093 | 4.9 × 10⁻⁴ | 195 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant K408A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant K408A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.03 | 6.9 × 10⁻⁴ | 50 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant K408A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant K440A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.112 | 4.8 × 10⁻⁴ | 270 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant K440A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant K440A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.088 | 7.8 × 10⁻⁴ | 113 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant K440A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N356A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.06 | 3.1 × 10⁻⁴ | 193 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N356A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N356A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.053 | 5.1 × 10⁻⁴ | 108 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N356A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N359A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.087 | 3 × 10⁻⁴ | 305 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N359A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N359A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.058 | 5.9 × 10⁻⁴ | 100 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N359A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N400A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.05 | 2.4 × 10⁻⁴ | 210 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N400A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N400A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.043 | 6.4 × 10⁻⁴ | 87 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N400A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N432A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.05 | 5.9 × 10⁻⁴ | 87 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N432A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant N432A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.04 | 0.0014 | 28 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant N432A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant Q362A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.117 | 4.6 × 10⁻⁴ | 275 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant Q362A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant Q362A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.045 | 0.00123 | 42 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant Q362A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant R434A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.033 | 8.3 × 10⁻⁴ | 45 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant R434A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant R434A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.103 | 4.4 × 10⁻⁴ | 247 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant R434A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant R439A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.118 | 9.5 × 10⁻⁴ | 150 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant R439A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant R439A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.1 | 0.00139 | 75 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant R439A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant S409E | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.05 | 2.8 × 10⁻⁴ | 180 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant S409E, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant S409E | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.017 | 4.9 × 10⁻⁴ | 42 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant S409E, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant T357A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.08 | 4.7 × 10⁻⁴ | 170 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant T357A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant T357A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.01 | 8.3 × 10⁻⁴ | 13 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant T357A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant Y405A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) | 0.082 | 3 × 10⁻⁴ | 283 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant Y405A, S. cerevisiae mitochondrial tRNAThr1 in vitro transcript (wild type) |
| mitochondrial threonyl-tRNA synthetase (MST1)variant Y405A | Saccharomyces cerevisiae | S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) | 0.06 | 9.4 × 10⁻⁴ | 70 | pH 7.2 | Open record: mitochondrial threonyl-tRNA synthetase (MST1) variant Y405A, S. cerevisiae mitochondrial tRNAThr2 in vitro transcript (wild type) |