CatLogenzyme kinetics catalog

Values CatLog read from this paper

Roles of amino acids in the Escherichia coli octaprenyl diphosphate synthase active site probed by structure-guided site-directed mutagenesis

PMID 22471615doi:10.1021/bi300069jOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (50 values) from this paper: octaprenyl diphosphate synthase (OPPS) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 16 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.5.1.90

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
octaprenyl diphosphate synthase (OPPS)Escherichia colifarnesyl diphosphate—0.0015—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)Escherichia coliisopentenyl diphosphate—0.004—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D211AEscherichia colifarnesyl diphosphate—0.0017—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D211AEscherichia coliisopentenyl diphosphate0.00750.02710.28 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D212AEscherichia colifarnesyl diphosphate—0.0031—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D212AEscherichia coliisopentenyl diphosphate0.00180.02330.077 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D215AEscherichia colifarnesyl diphosphate—0.0015—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D215AEscherichia coliisopentenyl diphosphate0.00870.01820.48 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D84AEscherichia colifarnesyl diphosphate—0.0092—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D84AEscherichia coliisopentenyl diphosphate1.2 × 10⁻⁴0.00930.013 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D85AEscherichia colifarnesyl diphosphate—0.024—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D85AEscherichia coliisopentenyl diphosphate3.2 × 10⁻⁴0.02940.011 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D88AEscherichia colifarnesyl diphosphate—0.0038—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant D88AEscherichia coliisopentenyl diphosphate1.1 × 10⁻⁴0.00850.013 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant H77AEscherichia colifarnesyl diphosphate—0.0242—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant H77AEscherichia coliisopentenyl diphosphate0.0190.10260.19 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K170LEscherichia colifarnesyl diphosphate—0.0032—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K170LEscherichia coliisopentenyl diphosphate3.3 × 10⁻⁴0.00490.067 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K225LEscherichia colifarnesyl diphosphate—0.0012—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K225LEscherichia coliisopentenyl diphosphate0.00430.00450.96 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K235LEscherichia colifarnesyl diphosphate—8 × 10⁻⁴—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K235LEscherichia coliisopentenyl diphosphate0.00960.00244.0 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K45AEscherichia colifarnesyl diphosphate—0.001—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant K45AEscherichia coliisopentenyl diphosphate0.00360.03350.11 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant Q208AEscherichia colifarnesyl diphosphate—0.0018—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant Q208AEscherichia coliisopentenyl diphosphate0.00190.07450.026 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant R48AEscherichia colifarnesyl diphosphate—0.0246—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant R48AEscherichia coliisopentenyl diphosphate0.00110.06390.017 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant R93AEscherichia colifarnesyl diphosphate—0.0052—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant R93AEscherichia coliisopentenyl diphosphate1.2 × 10⁻⁴0.00370.032 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant R94AEscherichia colifarnesyl diphosphate—0.0028—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant R94AEscherichia coliisopentenyl diphosphate8.4 × 10⁻⁵0.05390.0016 calc.pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant T171VEscherichia colifarnesyl diphosphate—8 × 10⁻⁴—pH 7.5, 25 °COpen
octaprenyl diphosphate synthase (OPPS)variant T171VEscherichia coliisopentenyl diphosphate0.00910.01130.81 calc.pH 7.5, 25 °COpen