CatLogenzyme kinetics catalog

Values CatLog read from this paper

Investigation of the role of Arg301 identified in the X-ray structure of phosphite dehydrogenase

PMID 22564138doi:10.1021/bi201691wOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 32 records (67 values) from this paper: phosphite dehydrogenase from Pseudomonas stutzeri, Methylobacterium extorquens, Nostoc punctiforme and 2 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.20.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
phosphite dehydrogenaseMethylobacterium extorquensNAD+—0.0405—pH 7.25, 30 °COpen
phosphite dehydrogenaseMethylobacterium extorquensphosphite5.10.23722 calc.pH 7.25, 30 °COpen
phosphite dehydrogenaseNostoc punctiformeNAD+—0.0194—pH 7.25, 30 °COpen
phosphite dehydrogenaseNostoc punctiformephosphite3.360.0254132 calc.pH 7.25, 30 °COpen
phosphite dehydrogenaseNostoc sp. PCC 7120NAD+—0.0103—pH 7.25, 30 °COpen
phosphite dehydrogenaseNostoc sp. PCC 7120phosphite2.410.0202119 calc.pH 7.25, 30 °COpen
phosphite dehydrogenasePseudomonas stutzeriNAD+—0.0522—pH 7.25, 30 °COpen
phosphite dehydrogenasePseudomonas stutzeriphosphite6.570.081181.0 calc.pH 7.25, 30 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriNAD+—0.022—pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite100 mM MOPS; NAD+ and phosphite both varied3.270.028117pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite100 mM MOPS; NAD+ saturating; phosphite varied3.260.0559pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphiteuniversal buffer (100 mM Tris, 50 mM MES, 50 mM acetic acid)2.710.09304pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite100 mM Tris-maleate; NAD+ saturating; phosphite varied2.930.3658pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336D/R301APseudomonas stutzeriphosphite100 mM MOPS0.03828.20.00135pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336D/R301APseudomonas stutzeriphosphiteuniversal buffer (100 mM Tris, 50 mM MES, 50 mM acetic acid)0.0386525.9 × 10⁻⁵pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336D/R301APseudomonas stutzeriphosphite100 mM Tris-maleate0.039497.9 × 10⁻⁴pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336D/R301APseudomonas stutzeriphosphite0.0055.29 × 10⁻⁴pH 5.5, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336D/R301APseudomonas stutzeriphosphite0.2549152.78 × 10⁻⁴pH 8.3, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/R301A/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriNAD+—0.216—pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/R301A/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite0.04119.40.00211pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/R301K/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriNAD+—0.074—pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/R301K/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite4.51.114.05pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/S295A/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriNAD+—0.25—pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/I150F/E175A/Q215L/R275Q/L276Q/S295A/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite2.530.10324.6pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/Y139F/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriNAD+—0.036—pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/Q137R/Y139F/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite3.680.03994.4pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/W134A/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriNAD+—0.187—pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/W134A/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite1.731.910.901pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/W134F/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriNAD+—0.102—pH 7.25, 25 °COpen
phosphite dehydrogenasevariant D13E/M26I/V71I/E130K/Q132R/W134F/Q137R/I150F/E175A/Q215L/R275Q/L276Q/I313L/V315A/A319E/A325V/E332N/C336DPseudomonas stutzeriphosphite2.960.23812.4pH 7.25, 25 °COpen
phosphite dehydrogenaseRalstonia metalliduransNAD+—0.0045—pH 7.25, 30 °COpen
phosphite dehydrogenaseRalstonia metalliduransphosphite6.830.0549124 calc.pH 7.25, 30 °COpen