Values CatLog read from this paper
A sialyltransferase mutant with decreased donor hydrolysis and reduced sialidase activities for directly sialylating LewisX
PMID 22583967doi:10.1021/cb300125kOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (51 values) from this paper: PmST1 from Pasteurella multocida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| PmST1 | Pasteurella multocida | 4-methylumbelliferyl beta-Lewisx [Galβ1-4(Fucα1-3)GlcNAcβ-] | 6.7 | 17 | 0.38 | pH 9.5, 37 °C | Open record: PmST1, 4-methylumbelliferyl beta-Lewisx [Galβ1-4(Fucα1-3)GlcNAcβ-] |
| PmST1 | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acid | 27 | 1.5 | 18 | pH 8.5, 37 °C | Open record: PmST1, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1 | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acid | 0.55 | 0.39 | 1.4 | pH 9.5, 37 °C | Open record: PmST1, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant D141A | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acid | 0.025 | 1.4 | 0.018 | pH 8.5, 37 °C | Open record: PmST1 variant D141A, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant H311A | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acid | 2.1 | 1.8 | 1.1 | pH 8.5, 37 °C | Open record: PmST1 variant H311A, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant M144D | Pasteurella multocida | 4-methylumbelliferyl beta-lactoside | 22 | 12 | 1.9 | pH 8.5, 37 °C | Open record: PmST1 variant M144D, 4-methylumbelliferyl beta-lactoside |
| PmST1variant M144D | Pasteurella multocida | 4-methylumbelliferyl beta-Lewisx [Galβ1-4(Fucα1-3)GlcNAcβ-] | 4 | 13 | 0.32 | pH 9.5, 37 °C | Open record: PmST1 variant M144D, 4-methylumbelliferyl beta-Lewisx [Galβ1-4(Fucα1-3)GlcNAcβ-] |
| PmST1variant M144D | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic aciddonor (CMP-Neu5Ac) hydrolysis activity; CMP-Neu5Ac 1-40 mM | 6.5 | 7.3 | 0.89 | pH 8.5, 37 °C | Open record: PmST1 variant M144D, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant M144D | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acidα2-3-sialyltransferase activity; Tris-HCl (100 mM, pH 8.5) | 1.9 | 0.3 | 6.1 | pH 8.5, 37 °C | Open record: PmST1 variant M144D, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant M144D | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acid | 0.59 | 2.1 | 0.28 | pH 9.5, 37 °C | Open record: PmST1 variant M144D, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant M144D | Pasteurella multocida | Neu5Acα2–3LacβMU | 0.035 | 20 | 0.0017 | pH 5.5, 37 °C | Open record: PmST1 variant M144D, Neu5Acα2–3LacβMU |
| PmST1variant M144H | Pasteurella multocida | 4-methylumbelliferyl beta-lactoside | 21 | 0.79 | 27 | pH 8.5, 37 °C | Open record: PmST1 variant M144H, 4-methylumbelliferyl beta-lactoside |
| PmST1variant M144H | Pasteurella multocida | 4-methylumbelliferyl beta-Lewisx [Galβ1-4(Fucα1-3)GlcNAcβ-] | 8.4 | 8.1 | 1 | pH 9.5, 37 °C | Open record: PmST1 variant M144H, 4-methylumbelliferyl beta-Lewisx [Galβ1-4(Fucα1-3)GlcNAcβ-] |
| PmST1variant M144H | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic aciddonor (CMP-Neu5Ac) hydrolysis activity; CMP-Neu5Ac 1-40 mM | 71 | 13 | 5.5 | pH 8.5, 37 °C | Open record: PmST1 variant M144H, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant M144H | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acidα2-3-sialyltransferase activity; Tris-HCl (100 mM, pH 8.5) | 21 | 0.81 | 27 | pH 8.5, 37 °C | Open record: PmST1 variant M144H, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant M144H | Pasteurella multocida | cytidine 5'-monophosphate-N-acetylneuraminic acid | 0.93 | 0.4 | 2.2 | pH 9.5, 37 °C | Open record: PmST1 variant M144H, cytidine 5'-monophosphate-N-acetylneuraminic acid |
| PmST1variant M144H | Pasteurella multocida | Neu5Acα2–3LacβMU | 0.027 | 1.7 | 0.016 | pH 5.5, 37 °C | Open record: PmST1 variant M144H, Neu5Acα2–3LacβMU |