CatLogenzyme kinetics catalog

Values CatLog read from this paper

Effects of N(2)-alkylguanine, O(6)-alkylguanine, and abasic lesions on DNA binding and bypass synthesis by the euryarchaeal B-family DNA polymerase vent (exo(-))

PMID 22793782doi:10.1021/tx300168pOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 54 records (162 values) from this paper: DNA polymerase Dpo4, DNA polymerase Vent (exo-) from Sulfolobus solfataricus, Thermococcus litoralis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.7

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphatetemplate G; unmodified G0.00280.880.0032pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphatetemplate N2-MeG; N2-MeG0.00270.790.0034pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphatetemplate N2-BzG; N2-BzG0.00160.590.0026pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphatetemplate O6-MeG; O6-MeG0.00362.20.0016pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphatetemplate O6-BzG; O6-BzG0.00121.20.001pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyadenosine 5'-triphosphatetemplate AP site; an abasic (tetrahydrofuran) site0.0211.10.02pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphatetemplate G; unmodified G0.380.01231pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphatetemplate N2-MeG; N2-MeG0.0920.004123pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphatetemplate O6-MeG; O6-MeG0.00550.0450.12pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphatetemplate O6-BzG; O6-BzG0.00570.0520.11pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphatetemplate AP site; an abasic (tetrahydrofuran) site0.00220.0270.083pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxycytidine 5'-triphosphatetemplate N2-BzG; N2-BzG0.00620.0262.4pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template G; Table 30.00450.430.01pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template N2-MeG0.00490.250.019pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template N2-BzG0.00160.050.03pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template O6-MeG3.2 × 10⁻⁴0.0120.027pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template O6-BzG5.5 × 10⁻⁴0.0770.0065pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template AP site0.00330.60.0055pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphateTable 40.0890.024.5pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphateTable 40.00260.390.0067pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricus2'-deoxyguanosine 5'-triphosphateTable 45.8 × 10⁻⁴0.390.0015pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricusthymidine 5'-triphosphatetemplate G; unmodified G0.0210.940.022pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricusthymidine 5'-triphosphatetemplate N2-MeG; N2-MeG0.0251.30.019pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricusthymidine 5'-triphosphatetemplate N2-BzG; N2-BzG1.9 × 10⁻⁴0.110.0016pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricusthymidine 5'-triphosphatetemplate O6-MeG; O6-MeG0.0633.80.017pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricusthymidine 5'-triphosphatetemplate O6-BzG; O6-BzG0.00230.870.0026pH 7.5, 37 °COpen
DNA polymerase Dpo4Sulfolobus solfataricusthymidine 5'-triphosphatetemplate AP site; an abasic (tetrahydrofuran) site0.0258.90.0028pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyadenosine 5'-triphosphatetemplate G; unmodified G0.00982.30.0043pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyadenosine 5'-triphosphatetemplate N2-MeG; N2-MeG0.00683.20.0021pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyadenosine 5'-triphosphatetemplate N2-BzG; N2-BzG0.00532.50.0022pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyadenosine 5'-triphosphatetemplate O6-MeG; O6-MeG0.00292.80.0011pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyadenosine 5'-triphosphatetemplate O6-BzG; O6-BzG0.00171.50.0011pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyadenosine 5'-triphosphatetemplate AP site; an abasic (tetrahydrofuran) site0.0430.430.099pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxycytidine 5'-triphosphatetemplate G; unmodified G0.0650.006211pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxycytidine 5'-triphosphatetemplate N2-MeG; N2-MeG0.0271.30.021pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxycytidine 5'-triphosphatetemplate N2-BzG; N2-BzG1 × 10⁻⁴2.44.2 × 10⁻⁵pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxycytidine 5'-triphosphatetemplate O6-MeG; O6-MeG0.0221.20.019pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxycytidine 5'-triphosphatetemplate O6-BzG; O6-BzG8 × 10⁻⁴0.130.0061pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxycytidine 5'-triphosphatetemplate AP site; an abasic (tetrahydrofuran) site0.00210.830.0025pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template G; Table 20.0121.20.01pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template N2-MeG0.00520.0840.062pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template N2-BzG6 × 10⁻⁴1.64 × 10⁻⁴pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template O6-MeG0.00672.30.0029pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template O6-BzG2 × 10⁻⁴0.316.4 × 10⁻⁴pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphatesingle-nucleotide insertion opposite template AP site0.0432.30.019pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphateTable 40.0420.0085.3pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphateTable 40.0071.10.0064pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralis2'-deoxyguanosine 5'-triphosphateTable 41.4 × 10⁻⁴0.0150.0093pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralisthymidine 5'-triphosphatetemplate G; unmodified G0.0432.70.016pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralisthymidine 5'-triphosphatetemplate N2-MeG; N2-MeG0.00211.80.0012pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralisthymidine 5'-triphosphatetemplate N2-BzG; N2-BzG4 × 10⁻⁴2.31.7 × 10⁻⁴pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralisthymidine 5'-triphosphatetemplate O6-MeG; O6-MeG0.0140.380.037pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralisthymidine 5'-triphosphatetemplate O6-BzG; O6-BzG0.0030.960.0031pH 7.5, 37 °COpen
DNA polymerase Vent (exo-)exo- (exonuclease-free form of Vent)Thermococcus litoralisthymidine 5'-triphosphatetemplate AP site; an abasic (tetrahydrofuran) site0.0172.10.0081pH 7.5, 37 °COpen