Values CatLog read from this paper
Engineering the acceptor specificity of trehalose phosphorylase for the production of trehalose analogs
PMID 22848048doi:10.1002/btpr.1609Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 42 records (126 values) from this paper: trehalose phosphorylase from Thermoanaerobacter brockii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| trehalose phosphorylase | Thermoanaerobacter brockii | D-galactose | 32.9 | 98 | 0.336 | pH 6, 60 °C | Open record: trehalose phosphorylase, D-galactose |
| trehalose phosphorylase | Thermoanaerobacter brockii | D-glucose | 33.9 | 3.2 | 11 | pH 6, 60 °C | Open record: trehalose phosphorylase, D-glucose |
| trehalose phosphorylasevariant A431T | Thermoanaerobacter brockii | D-galactose | 66.5 | 447.6 | 0.149 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A431T, D-galactose |
| trehalose phosphorylasevariant A431T | Thermoanaerobacter brockii | D-glucose | 32.1 | 36.8 | 0.872 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A431T, D-glucose |
| trehalose phosphorylasevariant A440E | Thermoanaerobacter brockii | D-galactose | 23 | 68.2 | 0.337 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440E, D-galactose |
| trehalose phosphorylasevariant A440E | Thermoanaerobacter brockii | D-glucose | 32.7 | 2.3 | 14 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440E, D-glucose |
| trehalose phosphorylasevariant A440K | Thermoanaerobacter brockii | D-galactose | 27.7 | 56.8 | 0.488 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440K, D-galactose |
| trehalose phosphorylasevariant A440K | Thermoanaerobacter brockii | D-glucose | 41.2 | 2.3 | 18 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440K, D-glucose |
| trehalose phosphorylasevariant A440M | Thermoanaerobacter brockii | D-galactose | 17 | 43.8 | 0.388 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440M, D-galactose |
| trehalose phosphorylasevariant A440M | Thermoanaerobacter brockii | D-glucose | 27.4 | 2.1 | 13 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440M, D-glucose |
| trehalose phosphorylasevariant A440V | Thermoanaerobacter brockii | D-galactose | 32.9 | 45.7 | 0.72 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440V, D-galactose |
| trehalose phosphorylasevariant A440V | Thermoanaerobacter brockii | D-glucose | 42 | 1.9 | 22 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440V, D-glucose |
| trehalose phosphorylasevariant A440V/N657Y | Thermoanaerobacter brockii | D-galactose | 23.6 | 35.1 | 0.672 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440V/N657Y, D-galactose |
| trehalose phosphorylasevariant A440V/N657Y | Thermoanaerobacter brockii | D-glucose | 16.1 | 0.6 | 27 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440V/N657Y, D-glucose |
| trehalose phosphorylasevariant A440V/R448S | Thermoanaerobacter brockii | D-galactose | 38.9 | 30.1 | 1.29 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440V/R448S, D-galactose |
| trehalose phosphorylasevariant A440V/R448S | Thermoanaerobacter brockii | D-glucose | 25 | 0.7 | 36 | pH 6, 60 °C | Open record: trehalose phosphorylase variant A440V/R448S, D-glucose |
| trehalose phosphorylasevariant N657D | Thermoanaerobacter brockii | D-galactose | 35.5 | 61.9 | 0.574 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657D, D-galactose |
| trehalose phosphorylasevariant N657D | Thermoanaerobacter brockii | D-glucose | 51.2 | 1.8 | 28 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657D, D-glucose |
| trehalose phosphorylasevariant N657G | Thermoanaerobacter brockii | D-galactose | 24 | 58.1 | 0.413 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657G, D-galactose |
| trehalose phosphorylasevariant N657G | Thermoanaerobacter brockii | D-glucose | 42.7 | 2.5 | 17 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657G, D-glucose |
| trehalose phosphorylasevariant N657I | Thermoanaerobacter brockii | D-galactose | 42.2 | 53.5 | 0.789 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657I, D-galactose |
| trehalose phosphorylasevariant N657I | Thermoanaerobacter brockii | D-glucose | 46.9 | 2.1 | 22 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657I, D-glucose |
| trehalose phosphorylasevariant N657L | Thermoanaerobacter brockii | D-galactose | 33.3 | 61.1 | 0.545 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657L, D-galactose |
| trehalose phosphorylasevariant N657L | Thermoanaerobacter brockii | D-glucose | 43.3 | 2.2 | 20 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657L, D-glucose |
| trehalose phosphorylasevariant N657R | Thermoanaerobacter brockii | D-galactose | 38.3 | 62.5 | 0.613 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657R, D-galactose |
| trehalose phosphorylasevariant N657R | Thermoanaerobacter brockii | D-glucose | 31.4 | 1.3 | 24 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657R, D-glucose |
| trehalose phosphorylasevariant N657Y | Thermoanaerobacter brockii | D-galactose | 23.3 | 45.8 | 0.509 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657Y, D-galactose |
| trehalose phosphorylasevariant N657Y | Thermoanaerobacter brockii | D-glucose | 53.9 | 3 | 18 | pH 6, 60 °C | Open record: trehalose phosphorylase variant N657Y, D-glucose |
| trehalose phosphorylasevariant P588H | Thermoanaerobacter brockii | D-galactose | 54.3 | 96 | 0.566 | pH 6, 60 °C | Open record: trehalose phosphorylase variant P588H, D-galactose |
| trehalose phosphorylasevariant P588H | Thermoanaerobacter brockii | D-glucose | 43.5 | 1.5 | 29 | pH 6, 60 °C | Open record: trehalose phosphorylase variant P588H, D-glucose |
| trehalose phosphorylasevariant R448D | Thermoanaerobacter brockii | D-galactose | 21.1 | 47.5 | 0.444 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448D, D-galactose |
| trehalose phosphorylasevariant R448D | Thermoanaerobacter brockii | D-glucose | 18.6 | 1.2 | 16 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448D, D-glucose |
| trehalose phosphorylasevariant R448F | Thermoanaerobacter brockii | D-galactose | 22.6 | 45.2 | 0.5 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448F, D-galactose |
| trehalose phosphorylasevariant R448F | Thermoanaerobacter brockii | D-glucose | 21.7 | 1.2 | 18 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448F, D-glucose |
| trehalose phosphorylasevariant R448N | Thermoanaerobacter brockii | D-galactose | 32.4 | 53.6 | 0.604 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448N, D-galactose |
| trehalose phosphorylasevariant R448N | Thermoanaerobacter brockii | D-glucose | 30.6 | 1.5 | 20 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448N, D-glucose |
| trehalose phosphorylasevariant R448S | Thermoanaerobacter brockii | D-galactose | 31.9 | 33.5 | 0.952 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448S, D-galactose |
| trehalose phosphorylasevariant R448S | Thermoanaerobacter brockii | D-glucose | 26.3 | 1.5 | 18 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448S, D-glucose |
| trehalose phosphorylasevariant R448S/N657Y | Thermoanaerobacter brockii | D-galactose | 14 | 38 | 0.368 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448S/N657Y, D-galactose |
| trehalose phosphorylasevariant R448S/N657Y | Thermoanaerobacter brockii | D-glucose | 7 | 0.6 | 12 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448S/N657Y, D-glucose |
| trehalose phosphorylasevariant R448V | Thermoanaerobacter brockii | D-galactose | 26.2 | 43.7 | 0.6 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448V, D-galactose |
| trehalose phosphorylasevariant R448V | Thermoanaerobacter brockii | D-glucose | 36.1 | 1.9 | 19 | pH 6, 60 °C | Open record: trehalose phosphorylase variant R448V, D-glucose |