Values CatLog read from this paper
Crystal structure of reaction intermediates in pyruvate class II aldolase: substrate cleavage, enolate stabilization, and substrate specificity
PMID 22908224doi:10.1074/jbc.m112.400705Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 5 records (13 values) from this paper: HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase | Escherichia coli | (S)-4-hydroxy-2-oxopentanoate | 361.5 | 0.35 | 1 × 10³ | pH 8 | Open record: HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase, (S)-4-hydroxy-2-oxopentanoate |
| HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase | Escherichia coli | (S)-4-hydroxy-2-oxopentanoate | 259 | 0.39 | 660 | Open record: HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase, (S)-4-hydroxy-2-oxopentanoate | |
| HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase | Escherichia coli | (S)-4-hydroxy-2-oxopentanoate | 0.69 | 3.4 | 0.20 calc. | pH 5 | Open record: HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase, (S)-4-hydroxy-2-oxopentanoate |
| HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase | Escherichia coli | 4-hydroxy-3-methyl-2-oxopentanoate | 14 | 0.05 | 280 calc. | pH 8 | Open record: HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase, 4-hydroxy-3-methyl-2-oxopentanoate |
| HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolasevariant R70K | Escherichia coli | (S)-4-hydroxy-2-oxopentanoate | 11.8 | 3.32 | 3.55 | pH 8 | Open record: HpaI (HpcH) 4-hydroxy-2-ketoheptane-1,7-dioate aldolase variant R70K, (S)-4-hydroxy-2-oxopentanoate |