Values CatLog read from this paper
Studies on the regioselectivity and kinetics of the action of trypsin on proinsulin and its derivatives using mass spectrometry
PMID 22982989doi:10.1016/j.bbapap.2012.09.004Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (24 values) from this paper: trypsin from Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| trypsin | Bos taurus | Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr | 0.3125 | 0.1115 | 3.2 | pH 8, 37 °C | Open record: trypsin, Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr |
| trypsin | Bos taurus | Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr-Arg-Arg | 4.15 | 0.00915 | 450 | pH 8, 37 °C | Open record: trypsin, Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Thr-Arg-Arg |
| trypsin | Bos taurus | human insulin (6 without N-terminal methionine) | 0.14 | 0.12 | 1.27 | pH 8, 37 °C | Open record: trypsin, human insulin (6 without N-terminal methionine) |
| trypsin | Bos taurus | human insulin with an additional N-terminal methionine | 0.14 | 0.12 | 1.3 | pH 8, 37 °C | Open record: trypsin, human insulin with an additional N-terminal methionine |
| trypsin | Bos taurus | human insulin with N-terminal methionine and B-chain C-terminal Arg31-Arg32 (di-arginyl M-insulin) | 1.1 | 0.0085 | 130 | pH 8, 37 °C | Open record: trypsin, human insulin with N-terminal methionine and B-chain C-terminal Arg31-Arg32 (di-arginyl M-insulin) |
| trypsin | Bos taurus | human proinsulin with an additional N-terminal methionine (recombinant M-proinsulin)MALDI-TOF MS | 11.65 | 0.115 | 102 | pH 8, 37 °C | Open record: trypsin, human proinsulin with an additional N-terminal methionine (recombinant M-proinsulin) |
| trypsin | Bos taurus | human proinsulin with an additional N-terminal methionine (recombinant M-proinsulin)RP-HPLC (220 nm) | 14.75 | 0.133 | 110 | pH 8, 37 °C | Open record: trypsin, human proinsulin with an additional N-terminal methionine (recombinant M-proinsulin) |
| trypsin | Bos taurus | N-alpha-benzoyl-L-arginine ethyl ester | 16.5 | 0.0153 | 1.1 × 10³ | Open record: trypsin, N-alpha-benzoyl-L-arginine ethyl ester |