CatLogenzyme kinetics catalog

Values CatLog read from this paper

Conferral of allostery to Thermus sp. GH5 methylglyoxal synthase by a single mutation

PMID 23038673doi:10.1093/jb/mvs115Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 15 records (19 values) from this paper: methylglyoxal synthase (TMGS) from Thermus sp. GH5.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.3.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
methylglyoxal synthase (TMGS)Thermus sp. GH5dihydroxyacetone phosphatephosphate 0 mM (allosteric inhibitor)326.40.5652.8pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)Thermus sp. GH5dihydroxyacetone phosphatephosphate 0.5 mM (allosteric inhibitor)279.80.6466.3pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)Thermus sp. GH5dihydroxyacetone phosphatephosphate 1 mM (allosteric inhibitor)233.2——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)Thermus sp. GH5dihydroxyacetone phosphatephosphate 1.5 mM (allosteric inhibitor)220.7——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97EThermus sp. GH5dihydroxyacetone phosphatephosphate 0 mM (allosteric inhibitor)4.5 × 10⁻⁴——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97EThermus sp. GH5dihydroxyacetone phosphatephosphate 0.5 mM (allosteric inhibitor)3.9 × 10⁻⁴——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97EThermus sp. GH5dihydroxyacetone phosphatephosphate 1 mM (allosteric inhibitor)2.5 × 10⁻⁴——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97EThermus sp. GH5dihydroxyacetone phosphatephosphate 1.5 mM (allosteric inhibitor)1.9 × 10⁻⁴——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97KThermus sp. GH5dihydroxyacetone phosphatephosphate 0 mM (allosteric inhibitor)0.7——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97KThermus sp. GH5dihydroxyacetone phosphatephosphate 0.5 mM (allosteric inhibitor)0.7——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97KThermus sp. GH5dihydroxyacetone phosphatephosphate 1 mM (allosteric inhibitor)0.5——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97KThermus sp. GH5dihydroxyacetone phosphatephosphate 1.5 mM (allosteric inhibitor)0.6——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant ΔR97Thermus sp. GH5dihydroxyacetone phosphatephosphate 0 mM (allosteric inhibitor)0.001——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant ΔR97Thermus sp. GH5dihydroxyacetone phosphatephosphate 0.2 mM (allosteric inhibitor)5.4 × 10⁻⁴——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant ΔR97Thermus sp. GH5dihydroxyacetone phosphatephosphate 0.5 mM (allosteric inhibitor)2.5 × 10⁻⁴——pH 6.5, 60 °COpen