Values CatLog read from this paper
Metabolic mechanism of mannan in a ruminal bacterium, Ruminococcus albus, involving two mannoside phosphorylases and cellobiose 2-epimerase: discovery of a new carbohydrate phosphorylase…
PMID 23093406doi:10.1074/jbc.m112.390336Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 43 records (109 values) from this paper: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) from Ruminococcus albus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | 3-Deoxy-3-fluoro-D-glucose | — | — | 0.0526 | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), 3-Deoxy-3-fluoro-D-glucose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | 3-O-Methyl-D-glucose | — | — | 0.0764 | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), 3-O-Methyl-D-glucose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | 4-O-beta-D-mannosyl-D-glucose | 83.3 | 0.304 | 274 calc. | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), 4-O-beta-D-mannosyl-D-glucose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | 6-Deoxy-6-fluoro-D-glucose | 42 | 156 | 0.269 | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), 6-Deoxy-6-fluoro-D-glucose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | 6-Deoxy-D-glucose | 177 | 119 | 1.49 | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), 6-Deoxy-D-glucose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | alpha-D-mannosyl phosphate | — | 5.8 | — | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), alpha-D-mannosyl phosphate |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | D-glucose | 204 | 38.2 | 5.34 calc. | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), D-glucose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | D-Glucose | 126 | 25.9 | 4.86 | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), D-Glucose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | D-Xylose | — | — | 0.18 | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), D-Xylose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | Laminaribiose | 16.6 | 156 | 0.106 | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), Laminaribiose |
| RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase) | Ruminococcus albus | phosphate | — | 5.34 | — | pH 6.5, 37 °C | Open record: RaMP1 (R. albus MP1, 4-O-beta-D-mannosyl-D-glucose phosphorylase), phosphate |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 1,5-Anhydro-D-glucitol | 20.7 | 79.2 | 0.261 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 1,5-Anhydro-D-glucitol |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 2-Deoxy-2-fluoro-D-glucose | 5.71 | 48.7 | 0.117 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 2-Deoxy-2-fluoro-D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 2-Deoxy-D-glucose | 11.7 | 90.7 | 0.129 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 2-Deoxy-D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 3-Deoxy-3-fluoro-D-glucose | 0.94 | 6.44 | 0.146 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 3-Deoxy-3-fluoro-D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 3-Deoxy-D-glucose | 0.412 | — | — | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 3-Deoxy-D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 3-O-Methyl-D-glucose | 0.763 | 7.82 | 0.0976 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 3-O-Methyl-D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 4-O-beta-D-mannosyl-D-glucosephosphorolysis of Man-Glc | 17.5 | 27.7 | 0.632 calc. | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 4-O-beta-D-mannosyl-D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | 4-O-beta-D-mannosyl-D-glucosephosphorolysis; Michaelis-Menten fit | 20.7 | 28.4 | 0.729 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), 4-O-beta-D-mannosyl-D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | alpha-D-mannosyl phosphate | — | 0.324 | — | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), alpha-D-mannosyl phosphate |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | Cellobiose | 20.2 | 22.2 | 0.91 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), Cellobiose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | D-Allose | 0.667 | 6.14 | 0.109 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), D-Allose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | D-glucose | 30.9 | 142 | 0.218 calc. | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), D-glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | D-Glucose | 34.5 | 172 | 0.201 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), D-Glucose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | D-Mannose | 5.12 | 45.6 | 0.112 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), D-Mannose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | Gentiobiose | 26.1 | 60.8 | 0.429 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), Gentiobiose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | Laminaribiose | 11.6 | 29.1 | 0.399 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), Laminaribiose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | Maltose | 5.07 | 50.4 | 0.101 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), Maltose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | methyl alpha-D-glucopyranoside | 20.1 | 85.3 | 0.236 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), methyl alpha-D-glucopyranoside |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | methyl beta-D-glucopyranoside | 35.2 | 85.2 | 0.413 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), methyl beta-D-glucopyranoside |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | N,N′-Diacetylchitobiose | 6.34 | 49.9 | 0.127 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), N,N′-Diacetylchitobiose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | N-Acetyl-D-glucosamine | 15.8 | 107 | 0.148 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), N-Acetyl-D-glucosamine |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | phosphate | — | 0.444 | — | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), phosphate |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | Sophorose | 13.8 | 49.8 | 0.277 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), Sophorose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannobiosesynthesis (reverse phosphorolysis); 50 mM MES-NaOH pH 6.5 | 45.1 | 30.7 | 1.47 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannobiose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannobiosephosphorolysis; cosubstrate inorganic phosphate | 7.06 | 44.5 | 0.159 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannobiose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannohexaose | 16 | 4.48 | 3.57 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannohexaose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannopentaosesynthesis (reverse phosphorolysis); 50 mM MES-NaOH pH 6.5 | 20.5 | 5.29 | 3.87 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannopentaose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannopentaosephosphorolysis; cosubstrate inorganic phosphate | 31.9 | 4.55 | 7.01 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannopentaose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannotetraosesynthesis (reverse phosphorolysis); 50 mM MES-NaOH pH 6.5 | 31.2 | 7.59 | 4.11 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannotetraose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannotetraosephosphorolysis; cosubstrate inorganic phosphate | 33.1 | 3.21 | 10.3 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannotetraose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannotriosesynthesis (reverse phosphorolysis); 50 mM MES-NaOH pH 6.5 | 34.6 | 13.3 | 2.6 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannotriose |
| RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase) | Ruminococcus albus | β-1,4-Mannotriosephosphorolysis; cosubstrate inorganic phosphate | 27.5 | 7.94 | 3.46 | pH 6.5, 37 °C | Open record: RaMP2 (R. albus MP2, beta-1,4-mannooligosaccharide phosphorylase), β-1,4-Mannotriose |