CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of the acid/base catalyst of a glycoside hydrolase family 3 (GH3) beta-glucosidase from Aspergillus niger ASKU28

PMID 23201198doi:10.1016/j.bbagen.2012.11.014Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 40 records (108 values) from this paper: beta-glucosidase (GH3) from Aspergillus niger.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.21

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
beta-glucosidase (GH3)Aspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside4220.49863pH 4, 40 °COpen
beta-glucosidase (GH3)Aspergillus niger2,4-dinitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit710.26273pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside0 mM sodium azide——197pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigercellobiose67.41.1160.6pH 4, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside1410.54263pH 4, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside0 mM sodium azide37.20.35105pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside5 mM sodium azide37.70.3898.2pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside10 mM sodium azide37.70.3997.6pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside30 mM sodium azide36.10.3796.7pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside50 mM sodium azide34.60.3891.4pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside200 mM sodium azide24.20.3274.6pH 6, 40 °COpen
beta-glucosidase (GH3)Aspergillus nigerp-nitrophenyl-beta-D-glucopyranoside500 mM sodium azide15.40.3148.7pH 6, 40 °COpen
beta-glucosidase (GH3)variant D463AAspergillus nigercellobiose78.4178.7pH 4, 40 °COpen
beta-glucosidase (GH3)variant D463AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside1600.64249pH 4, 40 °COpen
beta-glucosidase (GH3)variant D487AAspergillus nigercellobiose87.71.0385.2pH 4, 40 °COpen
beta-glucosidase (GH3)variant D487AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside1630.55297pH 4, 40 °COpen
beta-glucosidase (GH3)variant D496AAspergillus nigercellobiose78.30.57138pH 4, 40 °COpen
beta-glucosidase (GH3)variant D496AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside1410.44321pH 4, 40 °COpen
beta-glucosidase (GH3)variant D501AAspergillus nigercellobiose47.31.3634.7pH 4, 40 °COpen
beta-glucosidase (GH3)variant D501AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside1040.97107pH 4, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside2.120.121.2pH 4, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus niger2,4-dinitrophenyl-beta-D-glucopyranosideMichaelis-Menten non-linear fit0.340.01522.6pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside0 mM sodium azide——18.4pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside50 mM sodium azide——66.1pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside500 mM sodium azide——174.3pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside1000 mM sodium azide——217.1pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus niger2,4-dinitrophenyl-beta-D-glucopyranoside2000 mM sodium azide——249.2pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigercellobiose0.0011.020.001pH 4, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside0.326.090.05pH 4, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside0 mM sodium azide0.151.490.1pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside1 mM sodium azide0.899.80.09pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside2 mM sodium azide0.798.40.09pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside5 mM sodium azide0.585.720.1pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside10 mM sodium azide0.848.610.1pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside30 mM sodium azide0.889.260.09pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside50 mM sodium azide0.858.330.1pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside200 mM sodium azide0.877.920.11pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside500 mM sodium azide0.564.850.12pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside800 mM sodium azide0.423.520.12pH 6, 40 °COpen
beta-glucosidase (GH3)variant E490AAspergillus nigerp-nitrophenyl-beta-D-glucopyranoside1000 mM sodium azide0.727.010.1pH 6, 40 °COpen