Values CatLog read from this paper
Structural and mechanistic studies of HpxO, a novel flavin adenine dinucleotide-dependent urate oxidase from Klebsiella pneumoniae
PMID 23259842doi:10.1021/bi301262pOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 5 records (12 values) from this paper: HpxO (FAD-dependent urate oxidase) from Klebsiella pneumoniae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HpxO (FAD-dependent urate oxidase) | Klebsiella pneumoniae | NADH | — | 0.5 | 125 | pH 8, 25 °C | Open record: HpxO (FAD-dependent urate oxidase), NADH |
| HpxO (FAD-dependent urate oxidase) | Klebsiella pneumoniae | uric acid | 42 | 0.042 | 1 × 10³ | pH 8, 25 °C | Open record: HpxO (FAD-dependent urate oxidase), uric acid |
| HpxO (FAD-dependent urate oxidase)variant R204Q | Klebsiella pneumoniae | NADH | 0.26 | 0.64 | 0.41 calc. | pH 8, 25 °C | Open record: HpxO (FAD-dependent urate oxidase) variant R204Q, NADH |
| HpxO (FAD-dependent urate oxidase)variant Y216F | Klebsiella pneumoniae | NADH | — | 0.1 | 84 | pH 8, 25 °C | Open record: HpxO (FAD-dependent urate oxidase) variant Y216F, NADH |
| HpxO (FAD-dependent urate oxidase)variant Y216F | Klebsiella pneumoniae | uric acid | 8.4 | 0.09 | 93 | pH 8, 25 °C | Open record: HpxO (FAD-dependent urate oxidase) variant Y216F, uric acid |