Values CatLog read from this paper
Mutagenesis of a specificity-determining residue in tyrosine hydroxylase establishes that the enzyme is a robust phenylalanine hydroxylase but a fragile tyrosine hydroxylase
PMID 23368961doi:10.1021/bi400031nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 77 records (153 values) from this paper: tyrosine hydroxylase (TyrH) from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tyrosine hydroxylase (TyrH) | Rattus norvegicus | L-phenylalanine | 5.82 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH), L-phenylalanine |
| tyrosine hydroxylase (TyrH) | Rattus norvegicus | L-tyrosineDOPA formation; colorimetric assay; 400 µM 6-MePH4 | 3.33 | 0.04 | 83.3 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH), L-tyrosine |
| tyrosine hydroxylase (TyrH) | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 200 µM 6-MePH4; 60 µg/mL catalase | 3.98 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH), L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425A | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 5.22 | 0.021 | 247 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425A, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425A | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 11.92 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425A, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425A | Rattus norvegicus | L-tyrosine | 0.009 | 0.056 | 0.162 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425A, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425A | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 1.18 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425A, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425C | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.05 | 0.0152 | 68 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425C, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425C | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 1.67 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425C, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425C | Rattus norvegicus | L-tyrosine | 0.0055 | 0.015 | 0.37 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425C, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425C | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.503 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425C, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425E | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.27 | 0.045 | 28 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425E, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425E | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 2.08 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425E, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425E | Rattus norvegicus | L-tyrosineDOPA formation; colorimetric assay; 400 µM 6-MePH4 | 0.223 | 0.097 | 2.3 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425E, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425E | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 200 µM 6-MePH4; 60 µg/mL catalase | 1.98 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425E, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425F | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.98 | 0.0144 | 138 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425F, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425F | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 1.97 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425F, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425F | Rattus norvegicus | L-tyrosine | 0.002 | 0.0172 | 0.12 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425F, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425F | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.803 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425F, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425G | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.377 | 0.0053 | 307 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425G, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425G | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 3.5 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425G, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425G | Rattus norvegicus | L-tyrosine | 0.0067 | 0.0106 | 0.62 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425G, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425G | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.62 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425G, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425H | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | — | — | 2.3 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425H, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425H | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 1.78 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425H, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425H | Rattus norvegicus | L-tyrosine | 0.00112 | 0.0187 | 0.06 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425H, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425H | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.8 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425H, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425I | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 0.91 | 0.022 | 42 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425I, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425I | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 1.85 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425I, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425I | Rattus norvegicus | L-tyrosine | 0.0013 | 0.041 | 0.032 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425I, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425I | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.87 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425I, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425K | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.15 | 0.2 | 5.7 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425K, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425K | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 1.92 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425K, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425K | Rattus norvegicus | L-tyrosine | 0.0063 | 2.39 | 0.0027 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425K, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425K | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.7 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425K, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425L | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.193 | 0.019 | 45 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425L, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425L | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 3.2 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425L, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425L | Rattus norvegicus | L-tyrosine | 7 × 10⁻⁴ | 0.006 | 0.117 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425L, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425L | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.525 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425L, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425M | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 2.5 | 0.0164 | 157 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425M, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425M | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 4.65 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425M, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425M | Rattus norvegicus | L-tyrosine | 0.0045 | 0.0069 | 0.65 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425M, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425M | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 2.65 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425M, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425N | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 2.7 | 0.062 | 43 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425N, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425N | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 4.43 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425N, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425N | Rattus norvegicus | L-tyrosine | 0.077 | 0.172 | 0.445 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425N, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425N | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 1.95 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425N, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425Q | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 5.82 | 0.0565 | 103 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Q, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425Q | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 5.97 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Q, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425Q | Rattus norvegicus | L-tyrosineDOPA formation; colorimetric assay; 400 µM 6-MePH4 | 0.122 | 0.162 | 0.75 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Q, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425Q | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 200 µM 6-MePH4; 60 µg/mL catalase | 3.57 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Q, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425R | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.073 | 0.028 | 37 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425R, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425R | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 1.6 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425R, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425R | Rattus norvegicus | L-tyrosine | 3 × 10⁻⁴ | 0.088 | 0.0034 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425R, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425R | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.327 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425R, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425S | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 0.527 | 0.0092 | 57 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425S, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425S | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 1.49 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425S, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425S | Rattus norvegicus | L-tyrosine | 0.0168 | 0.0089 | 1.88 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425S, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425S | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 1.528 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425S, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425T | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 2.35 | 0.016 | 30 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425T, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425T | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 3.68 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425T, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425T | Rattus norvegicus | L-tyrosine | 0.0073 | 0.00105 | 7.05 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425T, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425T | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.73 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425T, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425V | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 1.463 | 0.0148 | 143 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425V, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425V | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 3.57 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425V, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425V | Rattus norvegicus | L-tyrosine | 6.3 × 10⁻⁴ | 0.04 | 0.0157 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425V, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425V | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 0.777 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425V, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425W | Rattus norvegicus | L-phenylalanine | 0.027 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425W, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425W | Rattus norvegicus | L-tyrosine | 0.053 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425W, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425Y | Rattus norvegicus | L-phenylalaninetyrosine formation; continuous assay at 275 nm | 0.693 | 0.0105 | 198 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Y, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425Y | Rattus norvegicus | L-phenylalanine6-MePH4 oxidation rate; 60 µg/mL catalase | 0.525 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Y, L-phenylalanine |
| tyrosine hydroxylase (TyrH)variant D425Y | Rattus norvegicus | L-tyrosine | 0.0025 | 0.0308 | 0.0807 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Y, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant D425Y | Rattus norvegicus | L-tyrosine6-MePH4 oxidation rate; 0.1 µM enzyme; 200 µM 6-MePH4 | 1.75 | — | — | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant D425Y, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant Q424A | Rattus norvegicus | L-tyrosine | 2.08 | 0.044 | 47 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant Q424A, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant Q426A | Rattus norvegicus | L-tyrosine | 2.4 | 0.066 | 37 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant Q426A, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant T427A | Rattus norvegicus | L-tyrosine | 2.9 | 0.051 | 57 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant T427A, L-tyrosine |
| tyrosine hydroxylase (TyrH)variant Y423A | Rattus norvegicus | L-tyrosine | 1.8 | 0.03 | 60 | pH 7, 30 °C | Open record: tyrosine hydroxylase (TyrH) variant Y423A, L-tyrosine |