CatLogenzyme kinetics catalog

Values CatLog read from this paper

Transmitting the allosteric signal in methylglyoxal synthase

PMID 23592737doi:10.1093/protein/gzt014Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 20 records (28 values) from this paper: methylglyoxal synthase (TMGS) from Thermus sp. GH5.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.2.3.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
methylglyoxal synthase (TMGS)Thermus sp. GH5dihydroxyacetone phosphateno phosphate3270.5653pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)Thermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)220——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant G56AThermus sp. GH5dihydroxyacetone phosphateno phosphate1.3 × 10⁻⁴——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant G56AThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)9.8 × 10⁻⁵——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant G56SThermus sp. GH5dihydroxyacetone phosphateno phosphate4.8 × 10⁻⁵——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant G56SThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)3.1 × 10⁻⁵——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97KThermus sp. GH5dihydroxyacetone phosphateno phosphate0.75——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant R97KThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)0.62——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant S55DThermus sp. GH5dihydroxyacetone phosphateno phosphate3002.76110pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant S55DThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)260——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant S55KThermus sp. GH5dihydroxyacetone phosphateno phosphate1——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant S55KThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)0.55——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant TMGS+Thermus sp. GH5dihydroxyacetone phosphateno phosphate1000.21490pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant TMGS+Thermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)76——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant V101AThermus sp. GH5dihydroxyacetone phosphateno phosphate3381.03330pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant V101AThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)240——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant V101IThermus sp. GH5dihydroxyacetone phosphateno phosphate0.75——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant V101IThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)0.37——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant V101SThermus sp. GH5dihydroxyacetone phosphateno phosphate1.4——pH 6.5, 60 °COpen
methylglyoxal synthase (TMGS)variant V101SThermus sp. GH5dihydroxyacetone phosphate1.5 mM phosphate (allosteric inhibitor)1.1——pH 6.5, 60 °COpen