Values CatLog read from this paper
Crystallographic analysis and structure-guided engineering of NADPH-dependent Ralstonia sp. alcohol dehydrogenase toward NADH cosubstrate specificity
PMID 23686719doi:10.1002/bit.24956Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (36 values) from this paper: RasADH (alcohol dehydrogenase) from Ralstonia sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| RasADH (alcohol dehydrogenase) | Ralstonia sp. | (R)-1-phenylethanol | 0.0047 | 5.5 | 8.5 × 10⁻⁴ | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase), (R)-1-phenylethanol |
| RasADH (alcohol dehydrogenase) | Ralstonia sp. | (S)-1-phenylethanolcosubstrate NAD+ | 0.0115 | 9.9 | 0.00115 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase), (S)-1-phenylethanol |
| RasADH (alcohol dehydrogenase) | Ralstonia sp. | (S)-1-phenylethanolcosubstrate NADP+ | 0.78 | 1.2 | 0.65 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase), (S)-1-phenylethanol |
| RasADH (alcohol dehydrogenase) | Ralstonia sp. | NADP+ | 1.65 | 0.051 | 32.3 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase), NADP+ |
| RasADH (alcohol dehydrogenase) | Ralstonia sp. | nicotinamide adenine dinucleotide | 0.022 | 3.1 | 0.007 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase), nicotinamide adenine dinucleotide |
| RasADH (alcohol dehydrogenase)variant G37D | Ralstonia sp. | NADP+ | 0.0025 | 2.3 | 0.00108 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase) variant G37D, NADP+ |
| RasADH (alcohol dehydrogenase)variant G37D | Ralstonia sp. | nicotinamide adenine dinucleotide | 0.038 | 2.3 | 0.017 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase) variant G37D, nicotinamide adenine dinucleotide |
| RasADH (alcohol dehydrogenase)variant N15G/G37D/R38V/R39S | Ralstonia sp. | (S)-1-phenylethanol | 0.0423 | 8.3 | 0.0052 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase) variant N15G/G37D/R38V/R39S, (S)-1-phenylethanol |
| RasADH (alcohol dehydrogenase)variant N15G/G37D/R38V/R39S | Ralstonia sp. | NADP+ | 2.8 × 10⁻⁵ | 0.28 | 1.02 × 10⁻⁴ | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase) variant N15G/G37D/R38V/R39S, NADP+ |
| RasADH (alcohol dehydrogenase)variant N15G/G37D/R38V/R39S | Ralstonia sp. | nicotinamide adenine dinucleotide | 0.08 | 1.1 | 0.073 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase) variant N15G/G37D/R38V/R39S, nicotinamide adenine dinucleotide |
| RasADH (alcohol dehydrogenase)variant N15G/G37D/R38V/R39S/A86N/S88A | Ralstonia sp. | (S)-1-phenylethanol | 0.023 | 17.6 | 0.0013 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase) variant N15G/G37D/R38V/R39S/A86N/S88A, (S)-1-phenylethanol |
| RasADH (alcohol dehydrogenase)variant N15G/G37D/R38V/R39S/A86N/S88A | Ralstonia sp. | nicotinamide adenine dinucleotide | 0.03 | 0.8 | 0.038 | pH 8.3, 30 °C | Open record: RasADH (alcohol dehydrogenase) variant N15G/G37D/R38V/R39S/A86N/S88A, nicotinamide adenine dinucleotide |