Values CatLog read from this paper
Structural determinants of specificity and catalytic mechanism in mammalian 25-kDa thiamine triphosphatase
PMID 23707715doi:10.1016/j.bbagen.2013.05.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (34 values) from this paper: 25-kDa thiamine triphosphatase from Mus musculus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 25-kDa thiamine triphosphatase | Mus musculus | inorganic tripolyphosphate | 1.6 | 0.29 | 5.5 calc. | 37 °C | Open record: 25-kDa thiamine triphosphatase, inorganic tripolyphosphate |
| 25-kDa thiamine triphosphatase | Mus musculus | thiamine triphosphate | 23 | 0.008 | 3 × 10³ | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant D147A | Mus musculus | thiamine triphosphate | 2.6 | 2.8 | 1 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant D147A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant D37A | Mus musculus | thiamine triphosphate | 2.8 | 0.2 | 14 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant D37A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant E81A | Mus musculus | thiamine triphosphate | 14.1 | 0.013 | 1 × 10³ | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant E81A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant K11A | Mus musculus | thiamine triphosphate | 6 | 0.012 | 500 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant K11A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant K193A | Mus musculus | thiamine triphosphate | 1.5 | 0.74 | 2 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant K193A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant K65A | Mus musculus | thiamine triphosphate | 0.019 | 0.009 | 2 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant K65A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant W53A | Mus musculus | inorganic tripolyphosphate | 2.2 | 0.097 | 23 calc. | 37 °C | Open record: 25-kDa thiamine triphosphatase variant W53A, inorganic tripolyphosphate |
| 25-kDa thiamine triphosphatasevariant W53A | Mus musculus | thiamine triphosphate | 4.4 | 0.55 | 8 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant W53A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant Y39A | Mus musculus | thiamine triphosphate | 0.64 | 0.62 | 1 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant Y39A, thiamine triphosphate |
| 25-kDa thiamine triphosphatasevariant Y79A | Mus musculus | thiamine triphosphate | 6 | 0.042 | 140 | pH 8.5, 37 °C | Open record: 25-kDa thiamine triphosphatase variant Y79A, thiamine triphosphate |