Values CatLog read from this paper
Engineering the meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum by site saturation mutagenesis for D-phenylalanine synthesis
PMID 23728814doi:10.1128/aem.01049-13Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: meso-diaminopimelate dehydrogenase (meso-DAPDH) from Symbiobacterium thermophilum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| meso-diaminopimelate dehydrogenase (meso-DAPDH) | Symbiobacterium thermophilum | phenylpyruvic acid | 0.11 | 12.5 | 0.0088 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH), phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant H227C | Symbiobacterium thermophilum | phenylpyruvic acid | 2.48 | 15.5 | 0.160 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant H227C, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant H227V | Symbiobacterium thermophilum | phenylpyruvic acid | 3.98 | 24.3 | 0.164 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant H227V, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant R181F | Symbiobacterium thermophilum | phenylpyruvic acid | 1.07 | 11.1 | 0.0964 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant R181F, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant R181F/H227V | Symbiobacterium thermophilum | phenylpyruvic acid | 3.43 | 20.8 | 0.165 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant R181F/H227V, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant T171P | Symbiobacterium thermophilum | phenylpyruvic acid | 0.53 | 15.8 | 0.034 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant T171P, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant T171S | Symbiobacterium thermophilum | phenylpyruvic acid | 0.88 | 19.6 | 0.045 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant T171S, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant T171S/H227V | Symbiobacterium thermophilum | phenylpyruvic acid | 2.37 | 13.9 | 0.171 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant T171S/H227V, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant T171S/R181F | Symbiobacterium thermophilum | phenylpyruvic acid | 0.7 | 13.9 | 0.050 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant T171S/R181F, phenylpyruvic acid |
| meso-diaminopimelate dehydrogenase (meso-DAPDH)variant T171S/R181F/H227V | Symbiobacterium thermophilum | phenylpyruvic acid | 2.09 | 11 | 0.19 calc. | pH 8.5, 30 °C | Open record: meso-diaminopimelate dehydrogenase (meso-DAPDH) variant T171S/R181F/H227V, phenylpyruvic acid |