Values CatLog read from this paper
Site-directed mutagenesis of catalytic residues in N(5)-carboxyaminoimidazole ribonucleotide synthetase
PMID 23899325doi:10.1021/bi400444yOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (58 values) from this paper: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) from Aspergillus clavatus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) | Aspergillus clavatus | 5-aminoimidazole ribonucleotide | 83.3 | 0.022 | 3.83 × 10³ | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase), 5-aminoimidazole ribonucleotide |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) | Aspergillus clavatus | adenosine 5'-triphosphate | 118.3 | 0.059 | 2 × 10³ | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase), adenosine 5'-triphosphate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) | Aspergillus clavatus | bicarbonate | 0.063 | 3.9 | 0.017 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase), bicarbonate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant D153A | Aspergillus clavatus | bicarbonate | 0.23 | 15 | 0.015 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant D153A, bicarbonate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant E73A | Aspergillus clavatus | bicarbonate | 0.25 | 33 | 0.0083 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant E73A, bicarbonate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant H273A | Aspergillus clavatus | 5-aminoimidazole ribonucleotide | 0.43 | — | — | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant H273A, 5-aminoimidazole ribonucleotide |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant H273A | Aspergillus clavatus | adenosine 5'-triphosphate | 0.38 | 0.175 | 2.2 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant H273A, adenosine 5'-triphosphate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant H273Q | Aspergillus clavatus | 5-aminoimidazole ribonucleotide | 0.0032 | 0.028 | 0.12 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant H273Q, 5-aminoimidazole ribonucleotide |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant H273Q | Aspergillus clavatus | adenosine 5'-triphosphate | 0.0033 | 0.095 | 0.033 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant H273Q, adenosine 5'-triphosphate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R155A | Aspergillus clavatus | bicarbonate | 0.143 | 7 | 0.017 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R155A, bicarbonate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R271A | Aspergillus clavatus | 5-aminoimidazole ribonucleotide | 0.0018 | 0.254 | 0.0083 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R271A, 5-aminoimidazole ribonucleotide |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R271A | Aspergillus clavatus | adenosine 5'-triphosphate | 0.00165 | 0.213 | 0.0083 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R271A, adenosine 5'-triphosphate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R271K | Aspergillus clavatus | 5-aminoimidazole ribonucleotide | 0.83 | 0.608 | 1.27 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R271K, 5-aminoimidazole ribonucleotide |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R271K | Aspergillus clavatus | adenosine 5'-triphosphate | 0.158 | 0.131 | 1.23 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R271K, adenosine 5'-triphosphate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R271K | Aspergillus clavatus | bicarbonate | 0.0013 | 80 | 1.7 × 10⁻⁵ | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R271K, bicarbonate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R271Q | Aspergillus clavatus | 5-aminoimidazole ribonucleotide | 0.0067 | 0.087 | 0.067 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R271Q, 5-aminoimidazole ribonucleotide |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant R271Q | Aspergillus clavatus | adenosine 5'-triphosphate | 0.0022 | 0.108 | 0.017 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant R271Q, adenosine 5'-triphosphate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant Y152A | Aspergillus clavatus | bicarbonate | 0.032 | 5 | 0.0067 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant Y152A, bicarbonate |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant Y152F | Aspergillus clavatus | 5-aminoimidazole ribonucleotide | 26.7 | 0.068 | 400 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant Y152F, 5-aminoimidazole ribonucleotide |
| N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase)variant Y152F | Aspergillus clavatus | bicarbonate | 0.053 | 2.8 | 0.017 | pH 7.8, 37 °C | Open record: N5-carboxyaminoimidazole ribonucleotide synthetase (N5-CAIR synthetase) variant Y152F, bicarbonate |