Values CatLog read from this paper
Molecular basis for N-terminal acetylation by the heterodimeric NatA complex
PMID 23912279doi:10.1038/nsmb.2636Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 28 records (54 values) from this paper: NatA complex (Naa10p·Naa15p), Naa10p (uncomplexed NatA catalytic subunit) from Schizosaccharomyces pombe.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 26 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Naa10p (uncomplexed NatA catalytic subunit) | Schizosaccharomyces pombe | NH2-EEEIAALRWGRPVGRRRRP-COOH | 0.19 | 1.72 | 0.11 calc. | pH 8, 25 °C | Open record: Naa10p (uncomplexed NatA catalytic subunit), NH2-EEEIAALRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p) | Schizosaccharomyces pombe | acetyl coenzyme A | — | 0.059 | — | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p), acetyl coenzyme A |
| NatA complex (Naa10p·Naa15p) | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 3 | 0.34 | 8.8 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p), NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant D532A (Naa15p subunit) | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.86 | 0.54 | 1.6 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant D532A (Naa15p subunit), NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant E24A | Schizosaccharomyces pombe | NH2-EEEIAALRWGRPVGRRRRP-COOH | 0.65 | 0.25 | 2.6 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant E24A, NH2-EEEIAALRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant E24D | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.42 | 0.75 | 0.56 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant E24D, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant E24Q | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.025 | 0.44 | 0.057 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant E24Q, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant E61A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 1.1 | 0.41 | 2.7 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant E61A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant E62A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 1.1 | 0.59 | 1.9 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant E62A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant F474A (Naa15p subunit) | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.4 | 0.35 | 1.1 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant F474A (Naa15p subunit), NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant F533A (Naa15p subunit) | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.9 | 0.33 | 2.7 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant F533A (Naa15p subunit), NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant F533A/F536A (Naa15p subunit) | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 3 | 0.5 | 6.0 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant F533A/F536A (Naa15p subunit), NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant F536A (Naa15p subunit) | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 1.8 | 0.45 | 4.0 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant F536A (Naa15p subunit), NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant H111A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 2 | 0.33 | 6.1 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant H111A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant H20A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 1.5 | 0.92 | 1.6 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant H20A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant H72A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 2.4 | 0.6 | 4.0 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant H72A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant K29A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 4.4 | 0.3 | 15 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant K29A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant K29A/Y33A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.61 | 0.34 | 1.8 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant K29A/Y33A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant K59A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 1.5 | 0.76 | 2.0 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant K59A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant K59A/E61A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 1.7 | 0.52 | 3.3 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant K59A/E61A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant K59A/E62A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.73 | 0.41 | 1.8 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant K59A/E62A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant L22A | Schizosaccharomyces pombe | acetyl coenzyme A | — | 0.053 | — | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant L22A, acetyl coenzyme A |
| NatA complex (Naa10p·Naa15p)variant L22A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.081 | 1.85 | 0.044 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant L22A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant P23A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 2 | 0.74 | 2.7 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant P23A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant R113A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.19 | 0.38 | 0.50 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant R113A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant R80A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.85 | 0.77 | 1.1 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant R80A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant Y26A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.047 | 0.73 | 0.064 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant Y26A, NH2-SASEAGVRWGRPVGRRRRP-COOH |
| NatA complex (Naa10p·Naa15p)variant Y33A | Schizosaccharomyces pombe | NH2-SASEAGVRWGRPVGRRRRP-COOH | 0.44 | 0.51 | 0.86 calc. | pH 8, 25 °C | Open record: NatA complex (Naa10p·Naa15p) variant Y33A, NH2-SASEAGVRWGRPVGRRRRP-COOH |