Values CatLog read from this paper
Directed evolution and structural analysis of NADPH-dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) reductase from Ralstonia eutropha reveals two mutations responsible for enhanced kinetics
PMID 23913421doi:10.1128/aem.01768-13Open in Browse
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CatLog read 6 records (15 values) from this paper: NADPH-dependent acetoacetyl-CoA reductase (PhaB) from Ralstonia eutropha.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NADPH-dependent acetoacetyl-CoA reductase (PhaB) | Ralstonia eutropha | acetoacetyl-CoA | 102 | 0.0057 | 1.8 × 10⁴ | pH 8, 30 °C | Open record: NADPH-dependent acetoacetyl-CoA reductase (PhaB), acetoacetyl-CoA |
| NADPH-dependent acetoacetyl-CoA reductase (PhaB) | Ralstonia eutropha | NADPH | — | 0.149 | 685 | pH 8, 30 °C | Open record: NADPH-dependent acetoacetyl-CoA reductase (PhaB), NADPH |
| NADPH-dependent acetoacetyl-CoA reductase (PhaB)variant Q47L | Ralstonia eutropha | acetoacetyl-CoA | 249 | 0.0159 | 1.52 × 10⁴ | pH 8, 30 °C | Open record: NADPH-dependent acetoacetyl-CoA reductase (PhaB) variant Q47L, acetoacetyl-CoA |
| NADPH-dependent acetoacetyl-CoA reductase (PhaB)variant Q47L | Ralstonia eutropha | NADPH | — | 0.289 | 862 | pH 8, 30 °C | Open record: NADPH-dependent acetoacetyl-CoA reductase (PhaB) variant Q47L, NADPH |
| NADPH-dependent acetoacetyl-CoA reductase (PhaB)variant T173S | Ralstonia eutropha | acetoacetyl-CoA | 361 | 0.0136 | 2.65 × 10⁴ | pH 8, 30 °C | Open record: NADPH-dependent acetoacetyl-CoA reductase (PhaB) variant T173S, acetoacetyl-CoA |
| NADPH-dependent acetoacetyl-CoA reductase (PhaB)variant T173S | Ralstonia eutropha | NADPH | — | 0.617 | 585 | pH 8, 30 °C | Open record: NADPH-dependent acetoacetyl-CoA reductase (PhaB) variant T173S, NADPH |