Values CatLog read from this paper
Incorporation of nucleoside probes opposite O⁶-methylguanine by Sulfolobus solfataricus DNA polymerase Dpo4: importance of hydrogen bonding
PMID 23959784doi:10.1002/cbic.201300296Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 7 records (14 values) from this paper: Dpo4 from Sulfolobus solfataricus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Dpo4 | Sulfolobus solfataricus | 2'-deoxycytidine 5'-triphosphateunmodified (G) template; with X = G | 0.28 | 0.0101 | 28 calc. | pH 7.4, 37 °C | Open record: Dpo4, 2'-deoxycytidine 5'-triphosphate |
| Dpo4 | Sulfolobus solfataricus | 2'-deoxycytidine 5'-triphosphateO6-methylguanine-containing template | 0.02 | 0.773 | 0.026 calc. | pH 7.4, 37 °C | Open record: Dpo4, 2'-deoxycytidine 5'-triphosphate |
| Dpo4 | Sulfolobus solfataricus | 2-thio-2'-deoxycytidine 5'-triphosphateunmodified (G) template; with X = G | 0.04 | 0.0668 | 0.60 calc. | pH 7.4, 37 °C | Open record: Dpo4, 2-thio-2'-deoxycytidine 5'-triphosphate |
| Dpo4 | Sulfolobus solfataricus | 2-thio-2'-deoxycytidine 5'-triphosphateO6-methylguanine-containing template | 0.03 | 0.98 | 0.031 calc. | pH 7.4, 37 °C | Open record: Dpo4, 2-thio-2'-deoxycytidine 5'-triphosphate |
| Dpo4 | Sulfolobus solfataricus | 5-methyl-2'-deoxycytidine 5'-triphosphateunmodified (G) template; with X = G | 0.33 | 0.0131 | 25 calc. | pH 7.4, 37 °C | Open record: Dpo4, 5-methyl-2'-deoxycytidine 5'-triphosphate |
| Dpo4 | Sulfolobus solfataricus | 5-methyl-2'-deoxycytidine 5'-triphosphateO6-methylguanine-containing template | 0.02 | 1.22 | 0.016 calc. | pH 7.4, 37 °C | Open record: Dpo4, 5-methyl-2'-deoxycytidine 5'-triphosphate |
| Dpo4 | Sulfolobus solfataricus | 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-beta-D-2'-deoxyribofuranosid-5'-triphosphate | 0.05 | 0.16 | 0.31 calc. | pH 7.4, 37 °C | Open record: Dpo4, 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-beta-D-2'-deoxyribofuranosid-5'-triphosphate |