Values CatLog read from this paper
Selective cleavage of glycyl bonds by papaya proteinase IV
PMID 2404797doi:10.1016/0014-5793(90)80101-nOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: papaya proteinase IV (PPIV), chymopapain, papain and 1 more from Carica papaya.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 3.4.22.2, EC 3.4.22.6, EC 3.4.22.25, EC 3.4.22.30
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| chymopapain | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide | 0.5 | 4.2 | 0.12 | pH 6.8, 20 °C | Open record: chymopapain, tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide |
| chymopapain | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin | 0.4 | 0.23 | 1.7 | pH 6.8, 30 °C | Open record: chymopapain, tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin |
| papain | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide | 1.6 | 1.4 | 1.14 | pH 6.8, 20 °C | Open record: papain, tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide |
| papain | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin | 2 | 0.14 | 14.3 | pH 6.8, 30 °C | Open record: papain, tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin |
| papaya proteinase III (PPIII) | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide | 0.5 | 4.2 | 0.12 | pH 6.8, 20 °C | Open record: papaya proteinase III (PPIII), tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide |
| papaya proteinase III (PPIII) | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin | 0.1 | 0.17 | 0.6 | pH 6.8, 30 °C | Open record: papaya proteinase III (PPIII), tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin |
| papaya proteinase IV (PPIV) | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide | 22 | 5.2 | 4.23 | pH 6.8, 20 °C | Open record: papaya proteinase IV (PPIV), tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 4-nitroanilide |
| papaya proteinase IV (PPIV) | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin | 5 | 0.16 | 31.2 | pH 6.8, 30 °C | Open record: papaya proteinase IV (PPIV), tert-butyloxycarbonyl-L-alanyl-L-alanyl-glycine 7-amido-4-methylcoumarin |
| papaya proteinase IV (PPIV) | Carica papaya | tert-butyloxycarbonyl-L-alanyl-L-alanyl-L-alanine 7-amido-4-methylcoumarin | 0.08 | 0.08 | 1 | pH 6.8, 30 °C | Open record: papaya proteinase IV (PPIV), tert-butyloxycarbonyl-L-alanyl-L-alanyl-L-alanine 7-amido-4-methylcoumarin |