Values CatLog read from this paper
C-di-GMP hydrolysis by Pseudomonas aeruginosa HD-GYP phosphodiesterases: analysis of the reaction mechanism and novel roles for pGpG
PMID 24066157doi:10.1371/journal.pone.0074920Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 6 records (12 values) from this paper: HD-GYP phosphodiesterase PA4781, HD-GYP phosphodiesterase PA4108 from Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HD-GYP phosphodiesterase PA4108 | Pseudomonas aeruginosa | 5'-phosphoguanylyl-(3'-5')-guanosine | 0.0012 | 0.03 | 0.040 calc. | pH 8, 30 °C | Open record: HD-GYP phosphodiesterase PA4108, 5'-phosphoguanylyl-(3'-5')-guanosine |
| HD-GYP phosphodiesterase PA4108 | Pseudomonas aeruginosa | cyclic 3'-5'-diguanylic acid | 1.5 × 10⁻⁴ | 0.02 | 0.0075 calc. | pH 8, 30 °C | Open record: HD-GYP phosphodiesterase PA4108, cyclic 3'-5'-diguanylic acid |
| HD-GYP phosphodiesterase PA4781 | Pseudomonas aeruginosa | 5'-phosphoguanylyl-(3'-5')-guanosine | 7.7 × 10⁻⁴ | 0.027 | 0.029 calc. | pH 8, 30 °C | Open record: HD-GYP phosphodiesterase PA4781, 5'-phosphoguanylyl-(3'-5')-guanosine |
| HD-GYP phosphodiesterase PA4781 | Pseudomonas aeruginosa | cyclic 3'-5'-diguanylic acid | 2 × 10⁻⁴ | 0.119 | 0.0017 calc. | pH 8, 30 °C | Open record: HD-GYP phosphodiesterase PA4781, cyclic 3'-5'-diguanylic acid |
| HD-GYP phosphodiesterase PA4781variant E314A | Pseudomonas aeruginosa | 5'-phosphoguanylyl-(3'-5')-guanosine | 2 × 10⁻⁴ | 0.017 | 0.012 calc. | pH 8, 30 °C | Open record: HD-GYP phosphodiesterase PA4781 variant E314A, 5'-phosphoguanylyl-(3'-5')-guanosine |
| HD-GYP phosphodiesterase PA4781variant E314A | Pseudomonas aeruginosa | cyclic 3'-5'-diguanylic acid | 6 × 10⁻⁴ | 0.0068 | 0.088 calc. | pH 8, 30 °C | Open record: HD-GYP phosphodiesterase PA4781 variant E314A, cyclic 3'-5'-diguanylic acid |