CatLogenzyme kinetics catalog

Values CatLog read from this paper

Alanine-scanning mutation approach for classification of the roles of conserved residues in the activity and substrate affinity of L-carnitine dehydrogenase

PMID 24101242doi:10.1007/s10529-013-1357-5Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (51 values) from this paper: L-carnitine dehydrogenase (Xt-CDH) from Xanthomonas translucens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 17 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.108

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
L-carnitine dehydrogenase (Xt-CDH)Xanthomonas translucensL-carnitine1.27910.20.125 calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)Xanthomonas translucensNAD+—0.31—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant E185AXanthomonas translucensL-carnitine0.2240.40.0054 calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant E185AXanthomonas translucensNAD+—0.36—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant E201AXanthomonas translucensL-carnitine0.003331.99.0 × 10⁻⁶ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant E201AXanthomonas translucensNAD+—0.35—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant F189AXanthomonas translucensL-carnitine0.009205.74.4 × 10⁻⁵ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant F189AXanthomonas translucensNAD+—1.04—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant F234AXanthomonas translucensL-carnitine0.00110569.5 × 10⁻⁷ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant F234AXanthomonas translucensNAD+—0.09—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant M231AXanthomonas translucensL-carnitine0.01308.83.2 × 10⁻⁵ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant M231AXanthomonas translucensNAD+—0.09—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant M246AXanthomonas translucensL-carnitine0.044336.21.3 × 10⁻⁴ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant M246AXanthomonas translucensNAD+—0.39—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant N144AXanthomonas translucensL-carnitine0.0791644.8 × 10⁻⁴ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant N144AXanthomonas translucensNAD+—0.35—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant Q252AXanthomonas translucensL-carnitine0.157101.40.00155 calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant Q252AXanthomonas translucensNAD+—0.4—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant R200AXanthomonas translucensL-carnitine0.001767.11.3 × 10⁻⁶ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant R200AXanthomonas translucensNAD+—2.25—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant R297AXanthomonas translucensL-carnitine0.016830.71.9 × 10⁻⁵ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant R297AXanthomonas translucensNAD+—0.26—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant S117AXanthomonas translucensL-carnitine0.019307.66.2 × 10⁻⁵ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant S117AXanthomonas translucensNAD+—3.76—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant S120AXanthomonas translucensL-carnitine0.0016671.5 × 10⁻⁶ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant S120AXanthomonas translucensNAD+—0.66—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant T262AXanthomonas translucensL-carnitine0.06889.17.6 × 10⁻⁴ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant T262AXanthomonas translucensNAD+—0.53—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant W228AXanthomonas translucensL-carnitine0.00810107.9 × 10⁻⁶ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant W228AXanthomonas translucensNAD+—0.07—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant Y147AXanthomonas translucensL-carnitine0.0072842.5 × 10⁻⁵ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant Y147AXanthomonas translucensNAD+—0.08—pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant Y237AXanthomonas translucensL-carnitine0.013799.71.6 × 10⁻⁵ calc.pH 9.5Open
L-carnitine dehydrogenase (Xt-CDH)variant Y237AXanthomonas translucensNAD+—0.39—pH 9.5Open