Values CatLog read from this paper
Protein engineering of α2,3/2,6-sialyltransferase to improve the yield and productivity of in vitro sialyllactose synthesis
PMID 24142961doi:10.1093/glycob/cwt092Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (72 values) from this paper: alpha2,3-sialyltransferase (α2,3PST), alpha2,6-sialyltransferase (α2,6PdST) from Pasteurella multocida, Photobacterium damselae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| alpha2,3-sialyltransferase (α2,3PST) | Pasteurella multocida | cytidine monophosphate N-acetylneuraminic acid | 12.6 | 1.73 | 7.27 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST), cytidine monophosphate N-acetylneuraminic acid |
| alpha2,3-sialyltransferase (α2,3PST) | Pasteurella multocida | lactose | 53.2 | 2.36 | 22.6 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST), lactose |
| alpha2,3-sialyltransferase (α2,3PST)variant R313H | Pasteurella multocida | cytidine monophosphate N-acetylneuraminic acid | 26.6 | 3.14 | 8.49 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313H, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,3-sialyltransferase (α2,3PST)variant R313H | Pasteurella multocida | lactose | 75.4 | 2.51 | 30.1 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313H, lactose |
| alpha2,3-sialyltransferase (α2,3PST)variant R313H/T265S | Pasteurella multocida | cytidine monophosphate N-acetylneuraminic acid | 44.6 | 4.09 | 10.9 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313H/T265S, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,3-sialyltransferase (α2,3PST)variant R313H/T265S | Pasteurella multocida | lactose | 73.9 | 1.64 | 45 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313H/T265S, lactose |
| alpha2,3-sialyltransferase (α2,3PST)variant R313N | Pasteurella multocida | cytidine monophosphate N-acetylneuraminic acid | 23.3 | 2.42 | 9.63 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313N, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,3-sialyltransferase (α2,3PST)variant R313N | Pasteurella multocida | lactose | 89.7 | 2.55 | 35.1 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313N, lactose |
| alpha2,3-sialyltransferase (α2,3PST)variant R313N/T265S | Pasteurella multocida | cytidine monophosphate N-acetylneuraminic acid | 36 | 2.91 | 12.6 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313N/T265S, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,3-sialyltransferase (α2,3PST)variant R313N/T265S | Pasteurella multocida | lactose | 82.1 | 2.14 | 38.4 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant R313N/T265S, lactose |
| alpha2,3-sialyltransferase (α2,3PST)variant T265S | Pasteurella multocida | cytidine monophosphate N-acetylneuraminic acid | 22.2 | 2.32 | 9.56 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant T265S, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,3-sialyltransferase (α2,3PST)variant T265S | Pasteurella multocida | lactose | 58.6 | 2.48 | 23.7 | pH 8.5 | Open record: alpha2,3-sialyltransferase (α2,3PST) variant T265S, lactose |
| alpha2,6-sialyltransferase (α2,6PdST) | Photobacterium damselae | cytidine monophosphate N-acetylneuraminic acid | 4.32 | 5.87 | 0.74 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST), cytidine monophosphate N-acetylneuraminic acid |
| alpha2,6-sialyltransferase (α2,6PdST) | Photobacterium damselae | lactose | 4.3 | 7.62 | 0.58 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST), lactose |
| alpha2,6-sialyltransferase (α2,6PdST)variant I411T | Photobacterium damselae | cytidine monophosphate N-acetylneuraminic acid | 10.9 | 6.06 | 1.8 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant I411T, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,6-sialyltransferase (α2,6PdST)variant I411T | Photobacterium damselae | lactose | 25.4 | 27.3 | 0.93 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant I411T, lactose |
| alpha2,6-sialyltransferase (α2,6PdST)variant I411T/L433S | Photobacterium damselae | cytidine monophosphate N-acetylneuraminic acid | 16.9 | 10.7 | 1.58 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant I411T/L433S, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,6-sialyltransferase (α2,6PdST)variant I411T/L433S | Photobacterium damselae | lactose | 102 | 75.7 | 1.35 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant I411T/L433S, lactose |
| alpha2,6-sialyltransferase (α2,6PdST)variant I411T/L433T | Photobacterium damselae | cytidine monophosphate N-acetylneuraminic acid | 18.8 | 3.37 | 5.6 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant I411T/L433T, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,6-sialyltransferase (α2,6PdST)variant I411T/L433T | Photobacterium damselae | lactose | 110 | 62 | 1.78 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant I411T/L433T, lactose |
| alpha2,6-sialyltransferase (α2,6PdST)variant L433S | Photobacterium damselae | cytidine monophosphate N-acetylneuraminic acid | 20.3 | 8.15 | 2.61 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant L433S, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,6-sialyltransferase (α2,6PdST)variant L433S | Photobacterium damselae | lactose | 85 | 59.7 | 1.43 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant L433S, lactose |
| alpha2,6-sialyltransferase (α2,6PdST)variant L433T | Photobacterium damselae | cytidine monophosphate N-acetylneuraminic acid | 10.7 | 2.27 | 4.7 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant L433T, cytidine monophosphate N-acetylneuraminic acid |
| alpha2,6-sialyltransferase (α2,6PdST)variant L433T | Photobacterium damselae | lactose | 76.5 | 61.1 | 1.26 | pH 8 | Open record: alpha2,6-sialyltransferase (α2,6PdST) variant L433T, lactose |