CatLogenzyme kinetics catalog

Values CatLog read from this paper

Molecular dynamics simulations and structure-guided mutagenesis provide insight into the architecture of the catalytic core of the ectoine hydroxylase

PMID 24184278doi:10.1016/j.jmb.2013.10.028Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 14 records (14 values) from this paper: ectoine hydroxylase (EctD) from Virgibacillus salexigens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.14.11.55

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
ectoine hydroxylase (EctD)Virgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—5.9—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant A163CVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—6—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant A163C/S244CVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—4.5—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant F242YVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—19.6—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant F263YVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—8—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant N261AVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—5.5—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant P198AVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—7.1—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant S165AVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—17.3—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant S167AVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—6.9—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant S205AVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—6.8—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant S244CVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—6.2—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant V265AVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—16.2—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant V265LVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—24.6—pH 7.5, 32 °COpen
ectoine hydroxylase (EctD)variant V265TVirgibacillus salexigens(4S)-2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid—9.7—pH 7.5, 32 °COpen