CatLogenzyme kinetics catalog

Values CatLog read from this paper

Improving glyphosate oxidation activity of glycine oxidase from Bacillus cereus by directed evolution

PMID 24223901doi:10.1371/journal.pone.0079175Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (48 values) from this paper: glycine oxidase (BceGO) from Bacillus cereus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 24 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.4.3.19

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
glycine oxidase (BceGO)Bacillus cereusglycine0.13621.040.131 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)Bacillus cereusN-phosphonomethylglycine0.095384.790.00112 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant D60GBacillus cereusglycine0.0760.990.077 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant D60GBacillus cereusN-phosphonomethylglycine0.119218.880.006310 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51RBacillus cereusglycine0.019354.63.54 × 10⁻⁴ calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51RBacillus cereusN-phosphonomethylglycine0.04928.290.00593 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60GBacillus cereusglycine0.007358.51.3 × 10⁻⁴ calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60GBacillus cereusN-phosphonomethylglycine0.04632.450.0189 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60G/K133R/V262IBacillus cereusglycine0.031105.62.9 × 10⁻⁴ calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60G/K133R/V262IBacillus cereusN-phosphonomethylglycine0.06382.770.0230 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60G/T118A/K133RBacillus cereusglycine0.022592.52.43 × 10⁻⁴ calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60G/T118A/K133RBacillus cereusN-phosphonomethylglycine0.06932.170.0320 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60G/T118A/K133R/I284LBacillus cereusglycine0.038341.649.21 × 10⁻⁴ calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/D60G/T118A/K133R/I284LBacillus cereusN-phosphonomethylglycine0.07720.570.14 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/D121G/S122P/H164Y/M267TBacillus cereusglycine0.094780.430.00118 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/D121G/S122P/H164Y/M267TBacillus cereusN-phosphonomethylglycine0.21651.370.158 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/I198V/E357GBacillus cereusglycine0.217101.80.00213 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/I198V/E357GBacillus cereusN-phosphonomethylglycine0.51333.80.14 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/K133R/S210P/R250G/V262IBacillus cereusglycine0.169138.10.00122 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/K133R/S210P/R250G/V262IBacillus cereusN-phosphonomethylglycine0.22031.690.130 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/S210P/M267TBacillus cereusglycine0.0902134.46.71 × 10⁻⁴ calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/S210P/M267TBacillus cereusN-phosphonomethylglycine0.12124.370.0277 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357GBacillus cereusglycine0.090541.550.00218 calc.pH 8.5, 25 °COpen
glycine oxidase (BceGO)variant G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357GBacillus cereusN-phosphonomethylglycine0.19450.530.37 calc.pH 8.5, 25 °COpen